The clarity of the paste or gel can vary from clear to opaque, and this property is related
to light dispersion resulting from the association of amylose and other components present in the starch (Karam, 2003). The increasing demand for new products has imposed to food industry the use of starches with characteristics such as absence of syneresis, transparency, stability and solubility to cold, which added to the restrictions on the use of chemically modified starches have directed researches for new sources of native starches with characteristics physico-chemical differentiated. The literature provides little information about the isolation and properties of starches from unconventional
sources such as fruit seeds. Studies on the functional properties Galunisertib solubility dmso of starch extracted from these seeds, including jackfruit seeds, have been conducted to verify its applicability in food, pharmaceutics and other uses and to replace with less costs commercial sources of starch (Aldana et al., 2011, Bello-Perez et al., 2006, Lawal and Adebowale, 2005 and Mukprasit and Sajjaanantakul, 2004). However, the jackfruit seeds could be found in soft and hard varieties, which have direct influences in properties of their starch. Still, climate and soil conditions, where the jackfruit is grown, could result in different chemical composition, consequently, have influence in functional properties (Aldana et al., 2011 and Bello-Perez et al., 2006). The present study characterised for the first time GDC-0199 in vivo starch extracted from two Brazilian jackfruit seeds varieties (hard and soft), focusing in the physicochemical, morphological and functional properties to determine its applicability in the food industry. Jackfruit seeds (A. heterophyllus L.) (soft and hard) were extracted from mature fruits purchased from a local market in João Pessoa city, Paraíba State, Brazil. The brown PLEKHB2 spermoderm covering the
cotyledons was removed by immersing jackfruit seeds in a 5% sodium hydroxide solution, followed by washing with running water. The starch was extracted from the cotyledons. The extraction of starch from hard and soft jackfruit seeds was conducted according to the slightly modified methodology of Loos, Hood, and Graham (1981). First, seeds were removed from pulp, peeled, cut into small pieces and allowed to soak for 24 h in a sodium metabisulphite solution (0.2%). Starch was extracted by grinding the raw material with sodium metabisulphite in a regular blender at low speed for 30 min. After homogenisation, the mixture was processed through a 200 mesh sieve (0.074 mm). The samples were then decanted twice for 24 h, with resuspension in sodium metabisulphite and centrifugation at 5000 rpm/15 min between each decanting; the supernatants of both were discarded.