Design: In 22 NICO patients, not having taken bisphosphonates, mu

Design: In 22 NICO patients, not having taken bisphosphonates, mutations affecting NO production (eNOS T-786C, stromelysin 5A6A) were measured by polymerase chain

reaction. Two healthy normal control subjects were matched per case by race and gender.

Results. Homozygosity for the mutant eNOS allele (TT) was present in 6 out of 22 patients (27%) with NICO compared with 0 out of 44 (0%) race and gender-matched control subjects; heterozygosity (TC) was present in 8 patients (36%) versus 15 control subjects (34%); and the wild-type normal genotype (CC) was present in 9 patients (36%) versus 29 controls (66%) (P = .0008). The mutant eNOS AICAR supplier T-786C allele was more common in cases (20 out of 44 [45%]) than in control subjects (15 out of 88 [17%]) (P = .0005). The distribution of the stromelysin 5A6A genotype in cases did not differ from control subjects (P = WH-4-023 research buy .13).

Conclusions. The eNOS T-786C polymorphism affecting NO production is associated with NICO, may contribute to the pathogenesis of NICO, and may open therapeutic medical approaches to treatment of NICO through provision of L-arginine, the amino-acid precursor of NO. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;

109: 548-553)”
“By 2015, it is estimated that nearly half of those living with HIV in the US will be 50 years of age and older. This dramatic change in the demographics of this clinical population represents unique challenges for patients, health care providers, and society-at-large. Fortunately, because of highly active antiretroviral therapy (HAART) and healthy lifestyle choices, it is now possible for many infected with selleck HIV to age successfully with this disease; however, this depends upon one’s definition of successful aging. It is proposed that successful aging is composed of eight factors: length of life, biological health, cognitive efficiency, mental health, social competence, productivity, personal control, and life satisfaction. Unfortunately, HIV and medication side effects can compromise these factors, thus diminishing one’s capacity to age successfully with this

disease. This article explores how HIV, medication side effects from HAART, and lifestyle choices can compromise the factors necessary to age successfully. Implications for practice and research are posited.”
“The prevalence of autism spectrum disorders for children with epilepsy in the general population is unknown. In a prospective community-based study of newly diagnosed childhood epilepsy, autism spectrum disorder was determined from parental interviews, medical records, and expert reviews by a child psychiatrist. A total of 28 (5%) participants had autism spectrum disorders. West syndrome (prevalence ratio = 4.53, P = .002) and intellectual impairment (prevalence ratio = 4.34, P = .002) were independently associated with autism spectrum disorder. Absent West syndrome, male gender was associated with autism spectrum disorder (prevalence ratio = 3.71, P = .

Results: Overall, 250 patients were evaluated No statistically s

Results: Overall, 250 patients were evaluated. No statistically significant difference between 16- and 18-gauge biopsy needles was recorded for cancer detection rate (29.6 vs. 30.4%, p = 0.890), core fragmentation rate (5 vs. 7%, p = 0.425) and shorter specimen (2 vs. 2%, p = 0.309).

Pain control was similar in the two groups during the biopsy, 30 min after biopsy and the evening of the same day. Very small or absent bleeding was the more frequent complication observed in each group. Conclusion: Prostate detection selleck screening library rate and sample quality were not influenced by needle size. A 16-gauge needle biopsy does not increase TRUS PBx morbidity. Copyright (C) 2012 S. Karger AG, Basel”
“Individual sugars, organic acids, total polyphenols, vitamin C and

antiradical activity (as measured by DPPH method) were quantified in cultivated Fragaria vesca berries, comparing different varieties (Regina delle Valli, Alpine, Sara and Valitutto) and different environments with regard to altitude. Cultivar effect mainly influenced the concentration of total polyphenols and antiradical activity which are strongly correlated LY3039478 in vivo (R(2)=0.91; P=0.001); conversely, altitude seemed to exert an influence in sugar and organic acid composition. The comparison of the general quality of the most diffused cultivar of F. vesca (Regina delle Valli and Alpine) evidenced that both cultivars have the same nutritional properties, whereas Regina delle Valli is better than Alpine from the point of view of total polyphenolic content (716 vs 471 mg catechin/100 g fresh weight) and radical scavenging activity (301 vs 219 ml DPPH solution/100 g fresh weight), thus resulting more attractive from the health protecting attributes point of view. F. vesca berries showed also a concentration of

sugars, citric acid, malic acid and total polyphenols much higher than those reported in literature for Fragaria x ananassa. (C) 2010 Elsevier Ltd. All rights reserved.”
“To evaluate the outcome of hysteroscopic myomectomy in patients with infertility and recurrent abortions.

This was a retrospective clinical Tipifarnib nmr analysis of 186 patients who underwent hysteroscopic myomectomy by monopolar electrode loop. A second-look diagnostic hysteroscopy was performed in all cases within 2 months. The mean follow-up period was 36.5 months.

The mean age of the patients was 28.4 years. The presenting clinical complaint was primary infertility in 31% (62/200) patients, secondary infertility in 10% (20/200) patients, recurrent abortions in 50.5% (101/200) and preterm deliveries in 8.5% (17/200) patients. Final reproductive outcome was assessed for 186 patients, after excluding patients with other confounding factors, interfering in pregnancy outcome.

There was a significant difference in reduction of number of miscarriages and increase in term deliveries while the number of preterm deliveries remained almost the same. Fifty-eight out of 82 infertile patients (70.

The median OS duration was 13 6 months in vemurafenib recipients

The median OS duration was 13.6 months in vemurafenib recipients and 9.7 months in dacarbazine recipients

in the most recent OS analysis.

In the phase III trial, progression-free survival (PFS) [co-primary endpoint] was also significantly improved in vemurafenib versus dacarbazine recipients (median PFS of 5.3 vs 1.6 months), with a significant reduction in the risk of death or disease progression of 74% in the final PFS analysis.

Vemurafenib was also associated with a high overall response rate in patients with previously treated, BRAF(V600) mutation-positive, Selleckchem Stem Cell Compound Library stage IV melanoma, according to the results of a noncomparative, multicenter, phase II trial. Patients had received at least one prior systemic treatment for advanced disease (excluding BRAF inhibitors other than sorafenib or MEK inhibitors). The overall response rate (primary endpoint) was 53% (complete response rate of 6% and partial response rate of 47%), with a median duration of response of 6.7 months, and a median OS duration of 15.9 months.

Oral vemurafenib AP26113 concentration was generally well tolerated in patients with metastatic melanoma, with cutaneous adverse events among the most commonly occurring adverse events. Cutaneous squamous cell carcinoma and/or keratoacanthoma were reported in 18% of vemurafenib recipients in the BRIM-3 trial.”

the perioperative phase, sepsis and sepsis-associated death are the most important problems for both the surgeon and the intensivist. Critically ill patients profit from an early identification and implementation of an interdisciplinary therapy. The purpose of this review on septic peritonitis this website is to give an update on the diagnosis and its evidence-based treatment.


diagnosis of sepsis is essential for patientA ‘ s survival. A bundle of studies was performed on early recognition and on new diagnostic tools for abdominal sepsis. Although surgical intervention is considered as an essential therapeutic step in sepsis therapy the time-point of source control is still controversially discussed in the literature. Furthermore, the Surviving Sepsis Campaign (SSC) guidelines were updated in 2012 to facilitate evidence-based medicine for septic patients.

Despite many efforts, the mortality of surgical septic patients remains unacceptably high. Permanent clinical education and further surgical trials are necessary to improve the outcome of critically ill patients.”
“The enzyme replacement therapy agalsidase alfa (Replagal (R)) has an amino acid sequence identical to that of native alpha-galactosidase A; intravenous agalsidase alfa 0.2 mg/kg every other week is indicated for the long-term treatment of patients with confirmed Fabry disease. This article reviews the efficacy and tolerability of agalsidase alfa in patients with Fabry disease, as well as summarizing its pharmacologic properties.

This work is prompted

This work is prompted this website by recent work on single-walled CNTs and UDD ensemble [Gupta et al., J. Appl. Phys. 107, 104308 (2010)] where radiation-induced microscopic defects seem to be stabilized by UDD. The present experiments show similar effects

where these hybrids display only a minimal structural modification under the maximum dose. Quantitative analyses of multiwavelength Raman spectra revealed lattice defects induced by irradiation assessed through the variation in prominent D, G, and 2D bands. A minimal change in the position of D, G, and 2D bands and a marginal increase in intensity of the defect-induced double resonant Raman scattered D and 2D bands are some of the implications suggesting the radiation coupling. The in-plane correlation length (L(a)) was also determined following Tunistra-Koenig relation buy IPI-145 from the ratio of D to G band (I(D)/I(G)) besides microscopic stress. However, we also suggest the following taking into account of intrinsic

defects of the constituents: (a) charge transfer arising at the interface due to the difference in electronegativity of MWCNT C sp(2) and UDD core (C sp(3)) leading to phonon and electron energy renormalization; (b) misorientation of C sp(2) at the interface of MWCNT and UDD shell (C sp(2)) resulting in structural disorder; (c) softening or violation of the q similar to 0 selection rule leading to D band broadening and a minimal change in G band intensity; and (d) normalized intensity of D and G bands Panobinostat cell line with 2D band help to distinguish defect-induced double resonance phenomena. The MWCNT when combined with nanodiamond showed a slight decrease in their conductance further affected by irradiation pointing at relatively good interfacial contact. Furthermore, owing to high thermal and electrical conductivity properties, they can facilitate potentially efficient heat-transfer applications and some results deduced

using Nielsen’s model is provided. (c) 2011 American Institute of Physics. [doi:10.1063/1.3524187]“
“The brittleness of poly(styrene maleic anhydride) (SMA) was improved by compositing with elastic poly(methyl methacrylate-butadiene-styrene) (MBS) to develop it into optical film. Transparent and flexible SMA films were prepared, and the flexibility of them was checked by folding endurance test and folding bend. The transparence of SMAMBS films were proved by the transmittance and haze measurements. In actual view, the letters could be clearly observed while they were covered by prepared films. The thermal stability of them was confirmed by DSC and TGA analysis. These physical properties of films are comparable to that of polycarbonate which is widely used as optical film.

Trans-sphenoidal surgery can decompress the optical nerve or ocul

Trans-sphenoidal surgery can decompress the optical nerve or oculomotornerve as a therapeutic strategy, and support biopsy or further pathological diagnosis. However, the hormonal therapy should be emphasized both as diagnostic and therapeutic strategies. Conservative and tentative steroid treatment should be performed in preoperative period without acute nerve damage.”
“Organicinorganic composite proton exchange membranes (PEMs) are of interest in fuel cell applications because of potential benefits in conductivity, mechanical, and transport properties that may be imparted by the inorganic component. Our previous work showed that polymeric Panobinostat ic50 membranes based on blends of poly(vinylidene

fluoride) (PVDF) and cross-linked sulfonated acrylic polyelectrolytes (PE) compare favorably against the proton conductivity and mechanical properties of commercial perfluorosulfonic acid-based PEMs. One problem found in the previous study was that crystalline regions in homopolymers of PVDF interfered with the formation of proton conducting pathways by the PE component. In this study, we explore the ability to use proton-conductive

zirconium-based inorganic particles to improve conductivity in such PVDF/PE membranes. Three different particles were considered, namely, zirconium oxide, zirconium hydroxide sulfate, and zirconium hydrogen phosphate. Dispersion of particles in the polymer matrix was limited, resulting in severe Ilomastat ic50 aggregation at particle loadings above 5 wt %. Nevertheless, a general improvement in proton conductivity was evidenced in composite membranes with 0.5 to 1 wt % particle loadings. This beneficial effect was particularly noticeable in membranes manufactured from highly crystalline PVDF

homopolymers (7 to 14% increase). We propose that the surface of zirconium particles act to provide proton conducting pathways between PE regions that otherwise would become blocked due to PVDF crystallization. In addition to conductivity, composite membranes exhibited enhancement find more of tensile properties at identical particle loadings, especially in membranes containing more flexible PVDF:HFP copolymers, where a reinforcing stiffening effect was evident (19 to 22% elastic modulus increment). (C) 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012″
“Purpose: To quantify tumor blood flow by using contrast material-enhanced destruction-replenishment ultrasonography (US) to evaluate tumor response to different doses of an agent for antiangiogenic treatment in hepatoma-bearing mice, with histologic measurements of microvascular density (MVD) as the reference standard.

Materials and Methods: Experiments were approved by the regional animal care committee. Mice bearing subcutaneous H22 hepatoma were treated with different doses of thalidomide, 100 mg/kg in group B and 200 mg/kg in group C. Group A (control group) was treated with 0.5% carboxylmethylcellulose.

Analysis of all results shows that nitro polycyclic aromatic hydr

Analysis of all results shows that nitro polycyclic aromatic hydrocarbons eFT508 mw (PAHs) are definitely environmental genotoxic/mutagenic hazards and confirms that environmental aromatic nitration reactions lead to an increase in genotoxicity and mutagenicity properties. Particularly 1-NPy and 1,8-DNPy can be considered as human potential

carcinogens. They seem to be significant markers of the genotoxicity, mutagenicity, and potential carcinogenicity of complex PAHs mixtures present in traffic emission and industrial environment. In prevention of environmental carcinogenic risk 1-NPy and 1,8-DNPy must therefore be systematically analyzed in environmental complex mixtures in association with combined umuC test, CBMN assay, and FISH on cultured human lymphocytes. (C) 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.”
“Artemisia lavandulaefolia has been utilized in traditional medicines for the treatment of several diseases. In this study, we attempted to determine whether the essential oil and 1,8-cineole of A. lavandulaefolia induce apoptosis in KB cells. The oil and 1,8-cineole induced the cell death of KB cells as evidenced by the increased cell population in the sub-G1 phase, the appearance of condensed and/or fragmented nuclei, and the generation of a cleaved PARP product. The treatment of the cells with the oil also induced

changes in the mitochondrial level of Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. Furthermore, the oil increased the phosphorylation of extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase, and p38 MAPK, but 1,8-cineole activated pp38 and INCB018424 molecular weight pJNK at the same concentrations. These findings show that S3I-201 concentration the mitochondrial and MAPKs pathways might be involved in the oil-induced apoptosis, and may enhance our current understanding of the anticancer functions of the oil to a greater degree than 1,8-cineole.”
“In this paper, the microbial transformation of resibufogenin (1) by Curvularia lunata AS 3.4381 was investigated, and four transformed products were isolated and characterized as 3-epi-resibufogenin (2),

12-hydroxy-3-epi-resibufogenin (3), 12-oxo-16-hydroxy-3-epi-resibufogenin (4), and 12,15-epoxy-3-epi-bufalin-14,15-ene (5). Among them, 4 and 5 are new compounds, and isomerization, hydroxylation, and oxidation reactions in microbial transformation process were observed. Additionally, the cytotoxicities of transformed products (2-5) were also investigated.”
“Although there have been advances in the fields of surgery, radiotherapy, and chemotherapy of tongue cancer, the cure rates are still not substantially satisfactory. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the major pungent ingredient of hot chili pepper and has been reported to have an antitumor effect on many human cancer cell types. The molecular mechanisms of the antitumor effect of capsaicin are not yet completely understood.

There was no difference in the prevalence of hypertension (56 %),

There was no difference in the prevalence of hypertension (56 %), diabetes (35 %) or stroke/transient ischemic attack (16 %). The one-year mortality rate was 23 %, while 31 %

of patients were rehospitalized; the combined end point was 51 %. Enrollment in an HF clinic was independently associated with reduced risk of total mortality (hazard ratio [HR] 0.69 [95 % CI 0.51 to 0.90], P=0.008; number needed to treat for one year to prevent the occurrence of one event [NNT]=16), all-cause hospital readmission (HR 0.27 [95 % CI 0.21 to 0.36], P < 0.0001; NNT=4), and combined mortality or hospital readmission (HR 0.73 [95 % CI 0.60 to 0.89], P < 0.0015; NNT=5).DISCUSSION:

HF clinics are associated with reductions in rehospitalization and mortality in an unselected HF population, independent of whether they are Apoptosis Compound Library cell line academic- or community-based. Such clinics should check details be made widely available to the HF population.”
“The discovery of specific gene mutations, termed driver mutations, in different tumors has brought personalized medicine into the focus of cancer treatment. Targeted treatment agents generally are administered orally and have a tolerable adverse event profile; they have become widely used in both inpatient and outpatient settings. The approval of the selective BRAF inhibitor

vemurafenib (Zelboraf (R)) as first-line therapy of metastatic melanoma in Europe in February 2012 as well as the increasing use of MEK inhibitors within clinical trials confronts dermatologists and oncologists with a new spectrum of side effects. Knowledge of these possible adverse events and their management will be crucial

for optimized patient care. This article offers an overview of the most important adverse events of currently employed dermato-oncologic therapeutic agents.”
“Pacemaker generator replacement is a simple procedure that is mostly done on outpatient basis. Electrocautery is almost universally used for bleeding control and tissue dissection. Ventricular fibrillation (VF) caused by electrocautery in these procedures is a very uncommon occurrence and an underappreciated possibility with modern day devices. We report a case of monomorphic ventricular tachycardia induced by electrocautery in a patient with normal left ventricular ejection fraction during elective pacemaker generator change. ( PACE 2009; 32: 957-958)”

Studies have suggested that patients who have undergone the Fontan procedure experience lower functional health status and diminished exercise capacity compared with other children.OBJECTIVES:

To compare the functional health status of Fontan patients with and without siblings, assess whether there are any differences between Fontan patients and their siblings, and determine associated factors.

It has become apparent that skin manifestations are an important

It has become apparent that skin manifestations are an important part of the disease process that need to be evaluated, treated and controlled. Other extramuscular manifestations, such as calcinosis, are Selleckchem Selinexor particularly difficult to treat, and studies are aimed at trying to elucidate predictors.

Recent findings

Larger cohort studies have enabled work on predictors of disease course and severity to be carried out. These include new autoantibodies in JDM (p140, which appears to have an association with calcinosis and p155 with lipodystrophy), cytokine polymorphisms, which appear to be risk factors

for developing JDM or for developing complications such as calcinosis, and the clinical findings of persistent nailfold capillary changes that are associated with a chronic disease course. Tools for more detailed assessment of skin disease have been developed and evaluated.


Improved collection

of standardized clinical data regarding extramuscular manifestations of JDM should allow researchers to continue elucidating the prognostic factors in this rare disease and assist multicentred trials in the evaluation of different treatment options.”
“The results of recent studies suggest that metformin, in addition to its antihyperglycemic efficacy, may also attenuate neuroinflammation and directly act on the central nervous system. However, the molecular mechanisms by which metformin exerts its anti-inflammatory effects in the brain remain largely unknown. Adenosine-monophosphate-activated protein kinase (AMPK) activation BKM120 ic50 DAPT datasheet is the most well-known mechanism of metformin action. However,

some of the biological responses to metformin (e. g. the release of cytokines and the expression of arginase I or PGC-1 alpha) are not limited to AMPK activation but also are mediated by AMPK-independent mechanisms. This article reviews current evidence supporting the hypothesis that the shift of microglia toward alternative activation may underlie the beneficial effects of metformin observed in animal models of neurological disorders.”
“Pre-eclampsia (PE) and eclampsia remain enigmatic despite intensive research. Growing evidence suggests that placental oxidative stress (OS) is involved in the etiopathogenesis of pre-eclampsia. Reduced perfusion as a result of abnormal placentation was proposed to be responsible for placental OS in PE. However, placental OS was also observed in normal pregnancy. The exact differences and correlation of placental OS in PE and normal pregnancy remain elusive. In this review, we attempted to link both normal pregnancy and PE on the causes of placental OS and proposed a hypothesis that placental OS in normal pregnancy, plus the exploration of other placental and/or maternal factors, could provide a novel explanation of that in PE.

We find a novel state in which the network activity is simultaneo

We find a novel state in which the network activity is simultaneously localized in both maps. In this state, a fixed cue 4SC-202 mw presented to the network does not determine uniquely the location of the bump, i.e. the response is unreliable, with neurons not

always responding when their preferred input is present. When the tuned input varies smoothly in time, the neuronal responses become reliable and selective for the environment: the subset of neurons responsive to a moving input in one map changes almost completely in the other map. This form of remapping is a non-trivial transformation between the tuned input to the network and the resulting tuning curves of the neurons. The new state of the network could be related to the formation of direction selectivity in one-dimensional environments and hippocampal remapping. The applicability of the model is not confined to self-position representations; we show an instance of the network solving a simple delayed

discrimination task.”
“Background: Chronic cannabis use has been associated with memory deficits and a volume reduction of the hippocampus, but none of the studies accounted for different effects of tetrahydrocannabinol (THC) and cannabidiol (CBD).

Methods: Using a voxel based morphometry approach optimized for small subcortical structures (DARTEL) gray matter (GM) concentration and volume of the hippocampus were measured in 11 chronic recreational cannabis users and 13 healthy controls, and correlated with THC and CBD from hair analyses. GM volume was calculated by modulating selleck products Screening Library VBM using jacobian determinants derived from the spatial normalization.

Results: Cannabis users showed lower GM volume located in a cluster of the right anterior hippocampus (P(uncorr) = 0.002: effect size Cohen’s d = 1.34). In a regression analysis an inverse correlation of the ratio THC/CBD with the volume of the right hippocampus (P(uncorr) p < 0.001, Cohen’s d = 3.43) was observed. Furthermore Cannabidiol correlated positively with GM concentration (unmodulated VBM data), but not with GM volume (modulated VBM) in the bilateral

hippocampus (P = 0.03 after correction for hippocampal volume; left hippocampus Cohen’s d = 4.37 and right hippocampus 4.65).

Conclusions: Lower volume in the right hippocampus in chronic cannabis users was corroborated. Higher THC and lower CBD was associated with this volume reduction indicating neurotoxic effects of THC and neuroprotective effects of CBD. This confirms existing preclinical and clinical results. As a possible mechanism the influence of cannabinoids on hippocampal neurogenesis is suggested. (c) 2010 Elsevier Ireland Ltd. All rights reserved.”
“Transparent copolymers composed of hexa (allyl 4-hydroxybenzoate) cyclotriphosphazene (compound 1) and styrene as potential halogen-free, flame-retardant optical resins were prepared by radical copolymerization.

“Objective: To investigate the outcome of cochlear implant

“Objective: To investigate the outcome of cochlear implantation in children with postmeningitic deafness and to compare the outcomes in ossified and nonossified cochleas.

Study Design: A retrospective case note review of children with postmeningitic deafness requiring cochlear implantation.

Setting: Manchester Auditory Implant Centre-a tertiary referral center.

Patients: Children younger than 16 years with postmeningitic deafness.


Cochlear implantation to aid auditory habilitation.

Main Outcome Measure: Outcomes were measured using Categories of Auditory Performance Score and the Manchester Speech and Language Development Scale.

Results: Fifty-two children underwent C188-9 in vivo cochlear implantation. Patients were allocated to 2 groups-Group 1 with nonossified

cochleas and Group 2 with cochlear ossification. Group 1 comprised 25 children, of whom, 22 had Categories of Auditory Performance Score of 5 or higher. Nineteen of them were able to use simple phrases of 3 words or more on the Manchester Speech and Language Development Scale. Group 2 comprised 27 children. Fourteen had partial ossification with complete standard electrode insertion in all instances. Of the 13 children with gross ossification, 3 had scala vestibuli insertion, 7 had split electrode insertion and 3 had partial electrode insertion. In this group, 20 children had Categories of Auditory Performance Score of 5 or higher. Nineteen children were able to use simple phrases of 3 words or more on Manchester Speech and Language Development Scale.

Conclusion: Children with postmeningitic deafness benefit significantly from cochlear implantation. However, the audio-logic outcomes are difficult to predict, especially in the presence of cochlear ossification. Acquisition of speech and language in these children after cochlear implantation is possible even in the presence of gross RG7321 cochlear ossification.”
“Bartonella henselae, a small fastidious Gram-negative bacillus, is the causative agent of cat-scratch disease (CSD). Because of difficulty in isolating the organism, there has been no

report on its antibiotic susceptibility in Japan. We determined the minimal inhibitory concentrations (MICs) of eight antimicrobial agents against 32 isolates of B. henselae (31 from cats and one from a human in Japan) by the Etest method. MICs of all 32 isolates were < 0.016 mu g/ml for minocycline and ranged from a parts per thousand currency sign0.016 to 0.064 mu g/ml for erythromycin, clarithromycin, azithromycin, ceftriaxone, and amoxicillin. MICs ranges of ciprofloxacin and gentamicin were from 0.064 to 0.25 mu g/ml and from 0.5 to 3 mu g/ml, respectively. All isolated strains showed high susceptibility to minocycline and macrolides antibiotics, which are currently used in the primary treatment of CSD in Japan. Although in vitro result of B.