Acknowledgements We thank Patricia K. Lankford for Western-blot technical support and the helpful comments from anonymous reviewers for the revision of this manuscript. MK-0457 in vitro This work is sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory (ORNL), managed by UT-Battelle, LLC for the U. S. Department of Energy under Contract No. DE-AC05-00OR22725. The BioEnergy Science Center is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Electronic supplementary material Additional file 1: PPT The comparison of Z. mobilis Hfq protein with homologues from
other species. Domain and motif sites of Z. mobilis Hfq (A), E. coli Hfq (B), S. cerevisiae Sm B (D), and S. cerevisiae Lsm1 (E) proteins based on NCBI BlastP result as well as the alignment
for some bacterial GSK1120212 supplier hfq homologues (C) using ClustalW 2 http://www.ebi.ac.uk/Tools/clustalw2/index.html. Residues that are identical across the species are indicated by “”*”", and residues that are not identical but conserved in function across the species are indicated by “”:”". (PPT ) Additional file 2: PPT Map of plasmid vector pBBR3DEST42. The vector map of pBBR3DEST42 plasmid constructed to analyze gene over-expressing and complementation. Tc(R): Tetracycline resistance gene tet; Cm: chloramphenicol resistance gene cat. attR1 and attR2 are recombination sites allowing recombinational cloning of the gene of interest from an entry clone; ccdB is ccdB gene allowing negative selection of expression clones. (PPT ) Additional file 3: PPT Lsm proteins in S. cerevisiae are involved in multiple inhibitor
tolerance. S. cerevisiae strains were grown in CM with 2% glucose (CM + glucose) for wild-type BY4741 and the deletion mutants, CM with 2% glucose and 2% galactose minus uracil (CM + glucose + 2% MRIP galactose) for GST overexpression strains. Five-μL culture was then transferred into 250-μL CM broth in the Bioscreen plate. The growth differences of different deletion mutant strains were monitored by Bioscreen (Growth Curves USA, NJ) in CM + glucose at pH 5.5 (A), CM + glucose with 305 mM NaCl, pH 5.5 (B), 305 mM NaAc, pH 5.5 (C), 305 mM NH4OAc, pH 5.5 (D), and 305 mM KAc, pH 5.5 (E), 0.75 g/L vanillin, pH 5.5 (F), 1.5 g/L Selleckchem XAV-939 furfural, pH 5.5 (G), and 1.5 g/L HMF, pH 5.5 (H). The growth differences of different GST-over-expressing strains were monitored by Bioscreen (Growth Curves USA, NJ) in CM + glucose + 2% galactose at pH 5.5 (I), CM + glucose + 2% galactose with 305 mM NaCl, pH 5.5 (J), 305 mM NaAc, pH 5.5 (K), 305 mM NH4OAc, pH 5.5 (L), 305 mM KAc, pH 5.5 (M), 0.75 g/L vanillin, pH 5.5 (N), 1.5 g/L furfural, pH 5.5 (O), and 1.5 g/L HMF, pH 5.5 (P). Strains included in this study are listed in table 1. This experiment has been repeated at least three times with similar result. (PPT ) References 1.