Conclusion The results presented in this work demonstrate a clear, dose dependent cytotoxic and antiviral effect of resveratrol: cytotoxicity at high concentration of the drug both on normal and tumor cells. On the other hand at low concentration, the continuous presence in the culture medium is necessary for the drug to be effective. The target of RV is the replication of viral DNA; however further studies are required for the full elucidation of the inhibitory mechanism mediated by RV leading to
the abrogation of the viral DNA synthesis. This effect was demonstrated in the absence of significant cytotoxic effects induced by the https://www.selleckchem.com/products/pf-477736.html drug. Removal of RV at short time after infection does not have a significant effect on the Selleck Eltanexor production of viral progeny DNA and this suggests that the viral
penetration is not the main target of the drug. Therefore we may conclude that the RV dependent inhibition of the viral proliferation occurs at subsequent stages: possibly during translocation of the virion from cytoplasm to nucleus. Finally this work gives a further support to the possibility that RV may find a potential clinical use for the control of proliferative pathologies and/or as an antiviral drug. Acknowledgements Financial support by the Italian Ministry of Education and Sigma-Tau is acknowledged (grants check details to GR). The collaboration of Michela Di Nottia in performing some experiments is also acknowledged. The graphic elaboration of the figures by Riccardo Risuleo is also acknowledged. References
1. Tooze J, (Editor): Molecular biology of tumor viruses: DNA Tumor Viruses. second edition. Cold Spring Harbor Laboratory Press, New York, USA; 1982. 2. Howley PM, Livingston DM: Small DNA tumor viruses: large contributors to biomedical sciences. Virology 2009, 384: 256–9.CrossRefPubMed 3. Yaniv M: Small DNA tumour viruses and their contributions to our understanding of transcription control. Virology 2009, 384: 369–374.CrossRefPubMed 4. Moens U, Johannessen M: Human polyomaviruses and cancer: expanding triclocarban repertoire. J Dtsch Dermatol Ges 2008, 6: 704–708.CrossRefPubMed 5. Jiang M, Abend JR, Johnson SF, Imperiale MJ: The role of polyomaviruses in human disease. Virology 2009, 384: 266–73.CrossRefPubMed 6. zur Hausen H: Novel human polyomaviruses – re-emergence of a well known virus family as possible human carcinogens. Int J Cancer 2008, 123: 247–250.CrossRefPubMed 7. Khalili K, Sariyer IK, Safak M: Small tumor antigen of polyomaviruses: role in viral life cycle and cell transformation. J Cell Physiol 2008, 215: 309–319.CrossRefPubMed 8. Iacoangeli A, Melucci-Vigo G, Risuleo G: Mechanism of the inhibition of murine polyomavirus DNA replication induced by the ionophore monensin. Biochimie 2000, 82: 35–39.CrossRefPubMed 9.