25 Ombitasvir (ABT-267) is an HCV NS5A inhibitor dosed once daily.26 and 27 ABT-450 is an HCV NS3/4A protease inhibitor, identified by AbbVie and Enanta as a lead

compound for clinical development. ABT-450 is co-dosed with low-dose ritonavir, a CYP3A4 inhibitor, to achieve therapeutic exposures at lower doses and at once daily dosing frequency (the combination is denoted ABT-450/r).27 Ombitasvir www.selleckchem.com/products/ly2835219.html has picomolar potency and ABT-450 has nanomolar potency against HCV genotypes 1, 2, and 3 in vitro. Phase 2 and 3 studies have demonstrated high SVR rates and tolerability of combination regimens that include ABT-450/r and ombitasvir with or without ribavirin in genotype 1-infected patients. 17, 18, 21, 28, 29, 30 and 31 In this exploratory phase 2 study, we assessed the safety and efficacy of pegIFN-free regimens of ombitasvir and ABT-450/r with or without RBV in treatment-naïve adults with chronic HCV genotype 1, 2, or 3 infection. This was an open-label, sequential arm, multicenter, combination treatment phase

2 study. Patients were screened and enrolled at 15 sites in the United States from September 2011 to March 2012. Treatment-naïve adults age 18–65 years (inclusive) with a BMI ≥18 and <35 kg/m2 and general good health, who were chronically infected with HCV genotype 1, 2, or 3 without evidence of cirrhosis, were eligible. Absence of cirrhosis was based on documented buy Ribociclib results of a FibroTest score of ≤0.72 and Aspartate Aminotransferase to Platelet Ratio ≤2, or Fibroscan result of <9.6 kPa at screening, or a liver biopsy within the last 36 months. Cohorts enrolling HCV genotype 1-infected patients were required to include at least 5 patients with HCV subgenotype

1a infection and at least 2 patients with HCV subgenotype 1b infection. Cohorts enrolling Cepharanthine HCV genotype 2-infected patients were required to include at least 2 patients with HCV subgenotype 2a infection and at least 5 patients with HCV subgenotype 2b infection. Patients were excluded if they had HIV or hepatitis B co-infection, or if they previously used any investigational or commercially available anti-HCV agents. The study was performed in accordance with Good Clinical Practice guidelines and the Declaration of Helsinki, and was approved by the relevant institutional review boards and regulatory agencies. All patients provided written informed consent. Eligible patients received either ombitasvir and ABT-450/r with RBV (Arm 1) or ombitasvir and ABT-450/r without RBV (Arm 2) for 12 weeks. Each arm included a cohort of genotype 1-infected patients, a cohort of genotype 2-infected patients, and a cohort of genotype 3-infected patients. Arms were enrolled sequentially. Once a cohort within Arm 1 was fully enrolled, the cohort of the same genotype in Arm 2 began enrolling patients. Patients received ombitasvir 25 mg once daily and ABT-450/r 200/100 mg once daily. RBV dosing was weight-based (1000 mg or 1200 mg total daily divided into 2 daily doses).