Classification and features The 16S selleckchem rRNA gene sequence of P. abscessus strain 7401987T was compared with sequences deposited in the Genbank database, confirming the initial taxonomic classification. Figure 1 shows the phylogenetic neighborhood of P. abscessus in a 16S rRNA based tree. The bacterium was characterized in 2007. It was isolated in the Timone Hospital microbiology laboratory (Table 1). Figure 1 Phylogenetic tree highlighting the position of Phocaeicola abscessus strain 7401987T relative to bacteria included in the Prevotella, Bacteroides and Paraprevotella genera by comparison of 16S rRNA gene sequences. GenBank accession numbers are indicated … Table 1 Classification and general features of Phocaeicola abscessus strain 7401987T Cells are coccoid (0.3-0.6 ��m wide and 0.4-0.

9 ��m long) to rod-shaped (0.4-1.7 ��m wide and 1.2-6.5 ��m long) and motile by flagella in a lophotrichous arrangement. Optimal growth of strain 7401987T occurs at 37��C with range for growth between 30 and 37 ��C. Surface colonies on chocolate agar after 7 days incubation at 37 ��C under anaerobic conditions were white, circular, regular, smooth, shiny, convex and 1 mm in diameter. The isolate was asaccharolytic. Activities of acid phosphatase, naphthol-AS-BI-phosphohydrolase, N-acetyl-��-glucosaminidase, ��-fucosidase, ��-galactosidase, ��-galactosidase, ��-galactosidase 6-phosphate, ��-glucosidase, N-acetyl-��-glucosaminidase, alkaline phosphatase, leucyl glycine arylamidase and alanine arylamidase were detected. The fatty acid profile was characterized by the predominance of anteiso-C15:0 (28.

2%), C16:0 (18.0%), iso-C15:0 (12.3%) and iso-C17:0 3-OH (11.7%). The size and ultrastructure of cells were determined by negative staining transmission electron microscopy. (Figure 2). Cells are coccoid (0.3-0.6 ��m wide and 0.4-0.9 ��m long) to rod-shaped (0.4-1.7 ��m wide and 1.2-6.5 ��m long). Figure 2 Transmission electron microscopy of P. abscessus strain 7401987T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 200 ��m. Genome sequencing and annotation Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the order Bacteroidales and is part of study of the new species characterized in our laboratory.

A summary of the project information is shown in Table 2. The EMBL accession number is “type”:”entrez-nucleotide”,”attrs”:CAKQ01000000″CAKQ01000000 and consists of 39 contigs (�� Brefeldin_A 500 bp) and 9 scaffolds. Table 2 shows the project information and its association with MIGS version 2.0 compliance. Table 2 Project information Growth conditions and DNA isolation P. abscessus strain 7401987T, was grown anaerobically on chocolate agar at 37��C.