The viral pellet was resuspended in the medium with polybrene and AGS cells for 12 hours. After infection, the virus-containing medium with fresh medium was replaced for 24 hours. Cells expressing a high Ma of green fluorescent protein were isolated Flt Signaling by fluorescence activated cell sorting, and the Bev lkerung shares was extended to create the cell line AGS luc. Mice and xenografts. M Nnlich nu / nu Mice were kept in the vivarium of the First Affiliated Hospital, College of Medicine in a pathogen-free unit, under a 00:00 light / dark cycle and were fed ad libitum with food and water. The Mice Were injected subcutaneously in the right Achselh cave or the peritoneal cave With HepG2, AGS luc, luc or inoculated Huh7 cells.
For experiments with AGS luc luc and Huh7 cells was carried out in vivo bioluminescence imaging with an imaging system lumina. Fifteen minutes prior to imaging, the Mice with 150 mg / kg intraperitoneally by injecting BI 2536 luciferin. Images were collected and analyzed with the Living Image software. The controller The vehicle DMSO was 20%. Results AMN, NA, and average share Similar growth inhibition in vitro apoptotic properties Our previous studies have shown that inhibiting at an average numonafides and growth of three cancer cell lines, with force Similar to AMN and demonstrating selectivity of t Similar to the inhibition the growth of cancer cells normal cells. Here we have systematically studied the growth inhibition of numonafides AMN and in 11 cell lines derived from various types of cancer.
The results show that numonafides, and on average, inhibit the growth of cancer cells with a potency Similar to that of NMA, although a tendency for slightly less effective. Because: An average and potent inhibitors of gastric and liver cancer cell lines and because these cancers are h INDICATIVE malignancies with relatively few opportunities Behandlungsm lebensf hig pharmacologically, here, retrospective evaluation of the antitumor properties using AGS, Huh7 and HepG2 cell lines. We evaluated the in vitro effect of these compounds on cell proliferation and apoptosis. Were initially Highest AGS cells treated with different doses of Amn, NA and stained to determine the medium and DNA content.
AMN, NA, and only because AVERAGE AGS cells their DNA content in a dose- Hen ngigen way to increased, And all compounds obtained Hen the amount of DNA to 5 M, indicating that these compounds cause the G2 arrest, which is probably the result of DNA-Sch by the intercalation or inhibition of topoisomerase II by these compounds. Then Huh7 cells with AMN and treated numonafides for 24 hours and then found Were rbt to determine the index of apoptosis. The results show that the NMA, NA, and all because AVERAGE significant apoptosis at 5 and 10 m, with average increases AMN and much m Powerful than A at both doses. AMN, NA, and even average influence gene expression profiling in genetic analysis of cancer cell table on cancer cells with numonafides, AVERAGE, and NMA were treated conducted to identify the use and to compare the molecular mechanisms and signaling pathways, cell by treating these compounds influenced.
HepG2 cells were treated with AMN, average or AT 2 million treated in the night and Ver Changes in the H Height of about 25,000 transcripts were determined by the set of genes. AVERAGE, AMN, and AN Ver Change significantly above the level of 347, 199, 178 and transcripts, each with more than 1.5 times flight neoplasia. 13, No. 5, 2011 median effective and less toxic than AMN Liu et al. 455th The naked