The slice thickness was 6 mm with a 1.2 mm interslice gap (forty slices) with a FOV of 40 × 32 or 40 × 40 cm depending on the arm’s size. Scan time for both scans was 3 minutes and 18 seconds. The MRI images from each site were saved in a DICOM format on an optical disc and sent to a central imaging facility for analysis. The muscle CSA of the thigh and arm was determined by manually tracing the margins of the muscles (all muscle compartments find more were included) and the external margin of the bone (periosteal

border). The muscle CSA was obtained by subtracting the total bone area from total muscle area at pre- and post-training. Analyses were performed by the same investigator using public domain software – Image J 1.33u (National Institutes of Health, USA). CSA of two slices per site were determined with the mean of the two slices used for statistical analyses. The slices were selected from the Selleckchem AMN-107 mid-point of the thigh and the mid-point of the arm (just distal to the deltoid insertion). To ensure that the slices analyzed pre- and post-training were taken from the same section of the thigh, the slice tangentially to the femoral head was used as an anatomical marker (first slice) and then https://www.selleckchem.com/products/AZD1152-HQPA.html numbered slice-by-slice distally. Two images mid-thigh were selected from each subject

and their numbers recorded and used to locate the same slice during post-testing. The ninth and tenth axial slices of the thigh were selected for most subjects. The same procedure was used for the arm with the slice tangentially to the humeral head used as an anatomical marker (first slice). The twelfth and thirteenth axial slices of the arm were selected for most subjects. In two subjects, for which the number Farnesyltransferase of slices between the first slice and the pre-training selected slices didn’t match (different anatomical position) during pre- and post-testing, images from the pre-training were compared to the post-training scans until an identical anatomical match was found. Training Program Subjects

assigned to both the CI and DI groups performed the same exercises, number of sets and exercises, and repetitions per set during 8-week monitored training period. The CI group trained with 2-minute rest intervals between sets all 8-weeks, 6 days per week using 4 sets of 8-10 RM for each exercise. The exercises and training days included the following: Monday and Thursday (free-weight bench press, free-weight incline bench press, machine wide grip front lat pull down and machine seated row), Tuesday and Friday (free-weight front military press, dumbbell shoulder lateral raise, biceps barbell curl, alternating biceps curl with dumbbells, triceps extension on a pulley machine with a v-shaped handle and lying triceps extension with a barbell), and Wednesday and Saturday (free-weight back squat, leg extension machine, leg curl machine and abdominal crunch).