MK and BK received fellowships from the the Higher Education Comm

MK and BK received fellowships from the the Higher Education Commission of Pakistan and the Austrian Science Fund, respectively. Thanks to Juliane Mayerhofer for providing plant material from Madeira, Portugal and to Jürgen Mairhofer, Peter Prištas and Sigrid Husar for helpful tips and comments. Electronic supplementary material Additional file 1: Annotation of the open reading frames. A table with annotation details of the open reading frames of all plasmids GDC-0068 in vivo isolated in this study is shown. (PDF 21 KB)

Additional file 2: Alignment of replication proteins. The data provide an alignment of the replication proteins of pHW104, pHW126 and related plasmids. (PDF 18 KB) Additional file 3: The RepA-like protein of the E. tasmaniensis Et/99 chromosome diverges at its C-terminus from plasmid RepA proteins. The data provide an alignment of the RepA sequences of pHW66, pYe4449-1 and pUB6060 and the RepA-like gene of the E. tasmaniensis Et1/99 chromosome. (PDF 16 KB) Additional file 4: Primers used in this AG-881 ic50 study. The data provide the sequences of primers used in this study. (PDF 8 KB) Additional file 5: Accession

numbers of sequences retrieved from databases. This table provides the accession numbers of sequences retrieved from databases and used for construction of phylogenetic trees and alignments. (PDF 53 KB) References 1. Berge O, Heulin T, Achouak W, Richard C, Bally R, Balandreau J: Rahnella aquatilis , a nitrogen-fixing enteric bacterium associated with the rhizosphere of wheat and maize. Can J Microbiol 1991, 37:195–203.CrossRef 2. Heulin T, Berge O, Mavingui P, Gouzou L, Hebbar KP, Balandreau J: Bacillus polymyxa and Rahnella aquatilis , the dominant N 2 -fixing bacteria associated with wheat rhizosphere in French soils. Eur J Soil Biol 1994, 30:35–42. 3. Hashidoko Y, Itoh E, Yokota K, Yoshida T, Tahara S: Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties. Biosci Biotechnol Biochem 2002, 66:2474–2478.PubMedCrossRef 4. Cankar

K, Kraigher H, Ravnikar M, Rupnik M: Bacterial endophytes from seeds of Norway spruce ( Picea abies L. Karst). FEMS Microbiol Lett 2005, 244:341–345.PubMedCrossRef 5. Lindow SE, Desurmont C, Elkins R, McGourty G, Clark E, Brandl MT: Occurrence of indole-3-acetic acid producing bacteria on pear trees and their association with fruit russet. Phytopathology 1998, 88:1149–1157.PubMedCrossRef Sclareol 6. Rozhon WM, Petutschnig EK, Jonak C: Isolation and characterization of pHW15, a small cryptic plasmid from Rahnella genomospecies 2. Plasmid 2006, 56:202–215.PubMed 7. Niemi RM, Heikkilä MP, Lahti K, Kalso S, Niemelä SI: Comparison of methods for determining the numbers and species distribution of coliform bacteria in well water samples. J Appl Microbiol 2001, 90:850–858.PubMedCrossRef 8. Brenner DJ, Müller HE, Steigerwalt AG, Whitney AM, O’Hara CM, Kämpfer P: Two new Rahnella genomospecies that Copanlisib chemical structure cannot be phenotypically differentiated from Rahnella aquatilis .

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