Rite outgrowth, the average length L Of neurites per cell, neurite outgrowth average normalized by the average number of cell forts Tze mean branches per cell per cell, and the average liquid surface Of cell K Body. Each function is described in the relevant section in the F Is more VX-222 VCH222 detailed and completely Ndiger data for NRG1 and NGF set is treated well, in ergs Complementary Tables 1 and 2 are provided.Table 3 erg Complementary Table 10 additionally USEFUL comprehensive statistical analysis of eight cell function, an assessment of the level is cellular Linear function normally distributed, and the observed length Zusammenh Of each function to another as the Pearson correlation coefficient. Based on these analyzes generally, as in additional keeping shown.
3, shows a graphic map of the two Pearson correlation between the eight-dimensional cellular Tional functions in NGF-screens were NRG1 and high content imaging, that many of these cellular Are REN properties are highly correlated to the 400 compounds and treatments there are differences in The film profiles the world between NRG1 and NGF. However, we have also found that there are potentially informative relationship between cellular Ren properties and neurites. For example, in the case of NRG1-treated cells, the function of the average L Of neurite length to the percentage of cells with neurites important and correlated the number of processes per cell, but was m Strength correlates with the number of branches per cell measured.
In contrast, correlates the average L Of neurites per cell length at least the size E of the Zellk Rpers, suggesting that NRG1 induced neuritogenesis my Trise soma size E can be separated k. Since we found that the average length Length of neurites per cell function was significantly correlated positively with the dose and the NGF treatment and NRG1 was w We hlten this function as a substitute for NRG1 and NGF signaling for use in screening for basis of the image may need during the realization that perform the analysis of other characteristics k can different types of NRG1 signaling modulators. This feature, in our test, the well controlled The DMSO-752 for each treatment were consistent with background levels of neurite outgrowth and Ver Changes in cell number. The mean length Neuritenl Each series of treatments can be controlled DMSO was taken as the reference value.
For the n Chsten data display, the value was the average Neuritenl Normalized length in each well in the induction of NRG1 or NGF to the respective reference value. In the library of 400 known bioactive compounds, 51 compounds in a significant reduction in cell number or in the form of NRG1 or NGF treatment, such as by a threshold, the out less than 100 cells defined after two days of incubation. Although these compounds k Can additionally USEFUL Ph Have genotypes at lower concentrations, they were not considered further in the studies reported here. The remaining 349 compounds were tested in nine categories according to their activity Th classified with respect to controlled The DMSO and their characteristics with respect and NRG1 NGFinduced neurite outgrowth with a simple change of time from 2 for both molecules, the potentiated neurite outgrowth and 0.5 times for molecules that inhibit outgrowth. The numbers of bioactive compounds in each category based on the classification are shown in Fig. 4C. To identify specific inhibitors of NRG1 E