53, 1618, 1304, 1597, 3053, 1329, 1180, 1597, 1524, 1H-NMR: d 6.83e8.03, 1.29e1.56, 2.87e3.50, Anal. Calculated Bicalutamide Casodex for C24H23N5O6: C 60.37, H 4.86, N 14.67, Found: C 60.39, H 4.84, N, 14.68. Compound 12: Mp 392e394, E Yield 81.30%, 1694 IR cm1 3395, 1237, 1617, 1342, 1237, 1548, have been reported 1HChina. The model of antibiotic resistance to Weissella is currently poorly understood. Resistance patterns were more St Strains of Weissella sp observed fish, but only six St Strains were evaluated in this study. Here we describe the outbreaks of the h Hemorrhagic septic Chemistry by Weissella sp.

in three commercial rainbow trout in Brazil. In addition, we examined the m Resembled infection pathways of these bacteria in fish, evaluated antibiotic resistance of the pathogen, and calculated laboratory-specific epidemiological cut-off values using normalized resistance interpretation.
Second Materials and methods 2.1. Outbreaks three outbreaks of the h Hemorrhagic septic chemistry In three commercial rainbow trout were examined. On farms Networks 1 and 2, the fish were reared in circular tanks, may need during the concrete aceways Were in the yard 3 to. An outbreak of plague occurred w During the summer when the water temperature is usually above 17 8C. The main clinical symptoms were anorexia, lethargy, exophthalmia, ascites, and bleeding in the mouth, oral cavity, Chairs tongue and eyes. W During epidemics were diseased rainbow trout sampled at 4 8C in Bo Your ice, and immediately taken to the laboratory for bacteriological examination.
A total of 41 diseased fish were collected, including 10 3 of farm 1, farm 2 6, and 25 of the farm 2.2. Isolation and biochemical characterization For bacterial isolation, samples of brain, kidney, liver, and ascites fluid from diseased fish sampled for fa Aseptic is distributed on a sheep blood agar, and at 25 8C for 72 h Pure colonies were a Gramf Staining, catalase and oxidase tests for the presence of H Thermolysis and growth at different temperatures and on MacConkey agar. The St Strains were at 80 8C in brain heart broth held at 15% glycerol until use. Before the biochemical and molecular tests, the isolates were cultured on Rogosa & Sharpe agar at 25 8C man for 24 h. Biochemical characterization was performed using the Rapid ID32 Strep commercial kit. 2.3.
Molecular analysis of the amplification and phylogenetic tree construction and sequential Age of 16S rRNA gene were for nine Feeder Llig selected Hlten Isolates from the total number of isolates performed. Total DNA was prepared using the commercial kit DNeasy. 16S rRNA was amplified by PCR with primers verst for the universal C70 and B37 Are RKT, as described by Fox et al. The PCR products were purified using a Wizard PCR Preps kit and sequenced using preheating Rts and Rev Rts primers. The sequential reactions Ages were recorded with a terminator cycle sequencing kit BigDyeTM Age and run on an ABI 3730xl Genetic Analyzer. The sequences were then compared to sequences in the NCBI database using BLAST algorithm. The limit for the identification of a bacterial species was 98% identity t of the nucleotides of the 16S rRNA gene. The phylogenetic relatedness of the isolates was determined by comparison of 16S rRNA sequence analysis of the gene. The sequences