Significant differences at a high level of *P < 0.01 can be visualized in Figure 2. Therefore, besides all previous comments, it is important to remark the significant difference between the amount of GA in the SC when applied Lip related to its corresponding PA textile and between the two formulations and their corresponding textiles in the deep layers of the skin (dermis and receptor fluid). When GA was embedded into the cosmetotextiles, a marked reservoir effect was always induced. A lower degree of GA skin penetration was obtained in most skin layers compared with the results obtained for the formulation

application alone. A similar penetration profile was Inhibitors,research,lifescience,medical obtained for the textiles treated with GA in MM or Lip in the skin compartments, SC, epidermis, and even in the dermis. In the dry textiles, the different lipid structures of Lip and MM, which may induce different enhancement behaviours, were lost. Therefore, textiles embedded with different vehicles may Inhibitors,research,lifescience,medical be expected to play a similar reservoir role. It is important to note that GA was absent in the receptor fluid of both the Lip-treated textiles and

Axitinib datasheet MM-treated PA. GA was only detected Inhibitors,research,lifescience,medical in the MM-treated CO fabric and in a smaller percentage than that detected with the free formulations. A comparison between the two textiles shows that much higher global percutaneous absorption was observed in the CO than in the PA fabric. GA was present in greater amounts in all skin layers when CO cosmetotextiles were topically applied and even Inhibitors,research,lifescience,medical reached the receptor

fluid when applied through MM. As in the washing desorption process, it seems that PA has greater substantivity for GA than CO vehiculised either in MM or Lip, because lecithin has been reported to incorporate mainly on the surface of CO fibres, whereas interaction with PA occurs to a greater extent Inhibitors,research,lifescience,medical in the interior [27]. Therefore, it seems reasonable to predict a higher reservoir effect for PA textiles, promoting a lower percutaneous absorption of GA. 4. Conclusions The precise amount of active agents present in cosmetotextiles was determined before from being used in a textile drug delivery system. Much greater absorption of the formulations was found for the MM treatments relative to that observed for the Lip treatments. However, the MM-treated fabrics showed much higher desorption, leading to a lower amount of absorbed material in the textile after washing. A large increase in particle size from 7 to 200nm was observed for MM, which indicates Lip formation due to surfactant dialysis. However, this increment in size does not help the formulation remain in the textile; on the contrary, it favours the desorption of the formulation. The percutaneous absorption of two formulations, Lip and MM, was evaluated, as was that of CO and PA textiles impregnated with the same Lip or MM.