Rats at 3 Weeks No. mammae with specified no. foci No. mammae Inoculum examined 0 1 2 3 4 5 6 7 None 50 12 17 6 5 6 2 1 1 FCA saline 49 15 16 4 3 5 3 2 1 FCA liver 48 17 14 8 3 4 2 0 0 FIA mamma 32 11 9 2 4 3 1 1 1 FCA mamma 75 7 15 12 9 7 5 4 16 FCA, Freund,s complete adjuvant, FIA, Freund,s incomplete XL880 Foretinib GSK1363089 adjuvant Chronological development of lesions The numbers of leucocytic foci in the hind mammae in control, oestrogenized rats and in those given an emulsion of Freund,s complete adjuvanlt and mammary homogenate are shown in Tables II and III. Though the control animal,s hindmammae occasionally contained 7 foci at 21 and 35 days, 8 or more foci were limited to the inoculated animals at 14, 21 and 28 days after inoculation.
Nilotinib In inoculated animals 7 or more foci were present in 6.3 of hind mammae at 14 days, 21 3 of hind mammae at 21 days, 25 0 of hind mammae at 28 days, and in 7.1 of hind mammae at 35 days. Macroscopically, the only change attributable to inoculation was purple discolouration of some mammae. This first appeared 21 days after inoculation, and was associated with increased vascularity and leucocytic infiltration in the mammae. Brown discolouration was unrelated to inoculation or to the inflammatory reaction. Microscopically, the first changes associated with inoculation of Freund,s complete adjuvant and mammary homogenate occurred at 14 days when tight perivenular exudates of mononuclear cells appeared close to small ducts and lobules. These cells were small round cells, morphologically either lymphoid or macrophages, granulocytes were scanty.
At 21 days the exudates were larger, many of the component cells possessed pyroninophilic cytoplasm, some early epithelial permeation was visible, and only a few granulocytes were present. The parenchyma was often condensed where the cellular infiltration had occurred. At 28 days the leucocytic foci had further increased in size, the proportion of histiocytes became larger and focal destruction of the parenchyma appeared. Thereafter the size and numbers of the foci declined, though some focal fibrosis was seen. Leucocytic foci in the control animals differed from the lesions described above in that they were looser knit, and showed more prominent granulocytes, particularly eosinophils, and histiocytes with conspicuously foamy cytoplasm.
Effect of site of inoculation Inoculation of mammary homogenate and Freund,s complete adjuvant did not evoke significantly different proportions of 7 or more leucocytic foci in the hind mammae when the sites on intradermal inoculation were altered. Seven or more foci were found in 21 3 of 75 hind mammae after inoculation at the base of the tail, 16.7 of 12 hind mammae after inoculation in the ipsilateral hind foot pad, and 15.4 of 26 hind mammae after inoculation in the ipsilateral front foot pad. Whilst the thoracic mammae, partly owing to interdigitation with local skeletal muscle, were too variable in their cross sectional area for quantitation of leucocytic responses, their reactions resembled those in the hind mammae after inoculations at the base of the tail. Effect of pertussis vaccine Inoculation of pertussis vaccine alone, or in combination with an injection of Freund,s complete adjuvant emulsified with s