In conclusion, this study describes a new approach for investigating neutrophil trafficking that can be used in preclinical studies to evaluate potential inhibitors of neutrophil recruitment. Polymorphonuclear (PMN) neutrophil transmigration across the mucosa and into intestinal crypts is a major characteristic of the inflammatory bowel diseases (IBD), Crohn’s disease (CD) and ulcerative colitis (UC). Excessive or unchecked neutrophil recruitment can lead to tissue damage, due mainly to the persistent release

of harmful inflammatory cytokines, reactive oxygen species and proteases by the infiltrated cells [1]. In active IBD, histological evidence of high-density neutrophil accumulation in the intestinal lumen https://www.selleckchem.com/products/abc294640.html correlates directly with epithelial injury and clinical disease activity [2]. Therefore, targeting neutrophil influx is a potential therapeutic strategy for IBD. The CXC chemokines, human interleukin-8 (IL-8/CXCL8) and the murine functional homologues keratinocyte-derived chemokine (KC/CXCL1) and macrophage inflammatory protein-2 (MIP-2/CXCL2), are neutrophil chemoattractants that orchestrate their activation and recruitment from the blood into sites of infection, inflammation and injury by promoting endothelial adhesion and transmigration [3]. Their biological effects are mediated by binding to two high-affinity

receptors, CXCR1 and CXCR2 [4]. CXCR2 has proved Epigenetics Compound Library to be a potent mediator Thymidylate synthase of PMN recruitment in preclinical models of arthritis [5], allergy [6], respiratory disease [7] and ulcerative colitis [8]. Increased mucosal expression of these chemokine receptors and their ligands in IBD explains the massive influx of leucocytes in active disease. The up-regulation of IL-8 in the colonic mucosa of IBD patients [9,10] correlates well with the histological degree of inflammation and chemokine mRNA expression

[11,12]. The pivotal involvement of keratinocyte-derived chemokine (KC) and macrophage inflammatory protein-2 (MIP-2) in PMN infiltration into inflammatory sites is also well documented [13,14]. Furthermore, a marked increase in KC and MIP-2 have been reported in colons of mice with acute phase dextran sulphate sodium (DSS)-induced colitis [15]. Traditional methods used to track neutrophil recruitment, such as static histological analysis of fixed tissues following adoptive transfer of dye-labelled cells, do not provide temporal or spatial information within the physiological environment of lymphoid tissues [16]. While white cell scintigraphy has been used to study neutrophil migration in both preclinical and clinical IBD studies [17,18], there are well-recognised disadvantages associated with radiotracers including the adverse effect on cell viability, radioactive decay and poor resolution [19].