Figure 1 FungiQuant in silico coverage analysis using the relaxed criterion against 993 genera and 9 phyla, demonstrating broad-coverage. On the 18S rRNA gene-based phylogeny, each analyzed fungal phylum is annotated with its genus-level FungiQuant coverage based on the relaxed criterion. This is presented as a numerator (i.e., the number of covered genus for the phylum), a denominator (i.e., the number of genera eligible for sequence matching for the phylum), and the percentage of coverage. FungiQuant sensitivity against diverse fungal DNA We tested the sensitivity of FungiQuant against 69 clinical and environmental species from

seven subphyla in the laboratory. We showed that FungiQuant is 100% sensitive against these diverse species from Agaricomycotina (n = 22), Mucormycotina (n = 4), Pezizomycotina (n = 29), Pucciniomycotina (n=2), Saccharomycotina (n = 17), Taphrinomycotina (n = 1), and buy GSK1120212 Ustilaginomycotina (n = 1) (Table 3). All of the fungal species tested were perfect sequence matches to FungiQuant, and based on results from three ten-fold dilutions, we found that the assay reaction efficiencies

ranged from 76.29% to 114.45%., with r 2 -value of >0.99 (Table 3). Table 3 FungiQuant sensitivity and reaction efficiency against click here diverse fungal species Subphylum Species Reaction efficiency r 2 Saccharomycotina Debaryomyces hansenii 101.42% >0.99 Saccharomycotina Lodderomyces Protein kinase N1 elongisporus 93.04% >0.99 Taphrinomycotina Schizosaccharomyces pombe 97.38% >0.99 Saccharomycotina Candida albicans 89.95% >0.99 Pezizomycotina Acremonium strictum 78.95% >0.99 Pezizomycotina Aspergillus flavus 85.96% >0.99 Pezizomycotina Aspergillus fumigatus 81.85% >0.99 Pezizomycotina Aspergillus niger 113.61% >0.99 Pezizomycotina Aspergillus versicolor 89.59% >0.99 Pezizomycotina Aureobasidium pullulans 84.08% >0.98 Pezizomycotina Chaetomium globosum 85.44% >0.99 Pezizomycotina Elaphomyces

decipiens 94.78% >0.99 Pezizomycotina Exophiala dermatitidis 76.29% >0.99 Pezizomycotina Fusarium equiseti 89.66% >0.99 Pezizomycotina Fusarium oxysporum 99.70% >0.98 Pezizomycotina Fusarium solani 103.38% >0.99 Pezizomycotina Microsporum canis 84.23% >0.99 Pezizomycotina Neurospora crassa 90.65% >0.99 Pezizomycotina Paecilomyces lilacinus 90.69% >0.99 Pezizomycotina Paecilomyces sinensis 82.30% >0.99 Pezizomycotina Paecilomyces variotii 95.15% >0.99 Pezizomycotina Penicillium marneffei 96.54% >0.99 Pezizomycotina Scedosporium apiospermum 91.58% >0.99 Pezizomycotina Sporothrix schenckii 90.86% >0.99 Pezizomycotina Trichophyton mentagrophytes 92.82% >0.99 Pezizomycotina Trichophyton rubrum 91.43% >0.99 Saccharomycotina Candida famata 90.13% >0.99 Saccharomycotina Candida guilliermondii 82.24% >0.99 Saccharomycotina Candida haemulonii 99.82% >0.99 Saccharomycotina Candida intermedia 81.72% >0.99 Saccharomycotina Candida quercitrusa 98.16% >0.99 Saccharomycotina Candida tropicalis 88.28% >0.