1% of total reads assigned in at least one of the samples).

All percentages are given as the percentage of total reads for each filtered metagenome. (DOC 88 KB) Additional file 3: Table S3. Reads assigned to archaeal taxa at the genus level in MEGAN (more than 0.1% of total reads assigned in at least one of the samples). All percentages are given as the percentage of total reads for each filtered metagenome. (DOC 33 KB) Additional RAD001 price file 4: Table S4. Reads length distribution for reads assigned at different taxonomic levels in MEGAN. (DOC 44 KB) Additional file 5: Table S5. Genomes used for KAAS annotation. (DOC 55 KB) References 1. Hornafius JS, Quigley D, Luyendyk BP: The world’s most spectacular marine hydrocarbon seeps (Coal Oil Point, Santa Barbara Channel, California): Quantification of emissions. J Geophys Res 1999,104(C9):20703–20711.CrossRef 2. Boles JR, Eichhubl P, Garven G, Chen J: Evolution of a hydrocarbon migration pathway along basin-bounding faults: Evidence from fault cement. Am Assoc Pet Geol Bull 2004,88(7):947–970. 3. Luyendyk B, Kennett J, Clark JF: Hypothesis for increased atmospheric methane input from hydrocarbon seeps on exposed continental shelves during glacial low sea level. Marine and Petroleum Geology 2005,22(4):591–596.CrossRef 4. Reeburgh WS: Oceanic methane biogeochemistry.

Chem Rev 2007,107(2):486–513.PubMedCrossRef 5. Reeburgh WS: ”Soft spots” in the Napabucasin order global methane budget. Microbial Growth on C1 Compounds 1996, 334–342.CrossRef 6. Niemann H, Lösekann T, de Beer D, Elvert M, Nadalig T, Knittel K, Amann R, Sauter EJ, Schlüter M, Klages M, et al.: Novel microbial communities of the Haakon Mosby mud volcano and their role as a methane sink. Nature 2006,443(7113):854–858.PubMedCrossRef 7. Knittel K, Lösekann T, Boetius A, Kort R, Amann R: Diversity and distribution of methanotrophic archaea at cold seeps. Appl Environ

Microbiol 2005,71(1):467–479.PubMedCrossRef 8. Hinrichs KU, Hayes JM, Sylva SP, Brewer PG, DeLong EF: Methane-consuming archaebacteria in marine sediments. Nature 1999,398(6730):802–805.PubMedCrossRef Dynein 9. Orphan VJ, Hinrichs KU, Ussler W, Paull CK, Taylor LT, Sylva SP, Hayes JM, Delong EF: Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments. Appl Environ Microbiol 2001,67(4):1922–1934.PubMedCrossRef 10. Boetius A, Ravenschlag K, Schubert CJ, Rickert D, Widdel F, Gieseke A, Amann R, Jørgensen BB, Witte U, Pfannkuche O: A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 2000,407(6804):623–626.PubMedCrossRef 11. Hallam SJ, Putnam N, Preston CM, Detter JC, Rokhsar D, Richardson PM, DeLong EF: Reverse methanogenesis: Testing the hypothesis with environmental genomics. Science 2004,305(5689):1457–1462.PubMedCrossRef 12.