The explorative approach of this study, combined with the finding of a decreased intracellular DCFH2 oxidation, revealed
an additional stimulation of cellular antioxidative mechanisms when exposed to CMH. This may contribute to an improved performance through increased ability to cope with training-induced Belnacasan nmr increases in oxidative stress. Combined effects of increased energy load and improved antioxidative defences may thus be the key to the performance improvement experienced by some athletes following creatine supplement, but this approach needs further investigation [41]. Acknowledgements The authors wish to thank Hanne S. Møller, Inge Lise Sørensen and Anne-Grete Dyrvig Petersen for excellent technical assistance. The Danish Technology and Production Research Council (FTP) is thanked for financial support through the project “”NMR-based metabonomics on tissues and biofluids”" (project no. 274-05-339). The Danish National Research Foundation and the Danish Biotechnological Instrument Centre (DABIC) is acknowledged. References 1. Balsom PD, Söderlund K, Ekblom B: Creatine in humans with special reference to creatine supplementation. Sports Med 1994, 18:268–280.CrossRefPubMed 2. Casey A, Constantin-Teodosiu D, Howell S, Hultman E, Greenhaff PL: Creatine ingestion favorably affects performance AG-014699 in vitro and muscle metabolism
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