The aim of this project is to identify cancer-related changes in the stroma during brain tumor progression that can be targeted therapeutically. However, targeting SC79 ic50 tumor-activated stromal cells require further insight into the mechanisms that regulate the tumor-stroma interplay. Since, any tumor biopsy contains a mixture of cancer cells and stromal cells, we are unable to

determine whether a given gene expression profile or protein signature is derived from stromal or cancer cells. For the same reason, we are also unable to specify the directions of cross-talk between compartments; whether an influence is excerted upon the tumor by the surrounding stroma, or vice versa. In this project, we have generated a green fluorescent protein (GFP) -expressing on the nude rat by crossing nude rat with a CA4P transgenic GFP-expressing line. We implant human glioma biopsies in green-fluorescent (GFP) immunodeficient rats. The resulting xenograft tumors are dissociated into a cell suspension and

FACS-sorted into GFP-positive stromal cells and GFP-negative tumor cells. We also obtained cell suspensions of stromal cells from normal brain. Human specific nuclei antibody staining has confirmed that sufficient purity of the sorted cells. Using this tool, we intend to delineate the gene expression profiles and protein signatures unique to the tumor-activated stromal cells. This information will subsequently be used to tailor drug regimens that target tumor-activated stroma and tumor-stroma Temsirolimus clinical trial interactions. O182 Does Hypoxia Play a Role in the Failure of Androgen Ablation Therapy for Prostate Cancer? Jenny Worthington 1 , Louise Ming1, Maxwell Omabe1, Christopher Mitchell1, Stephanie McKeown1 1 Biomedical Sciences Research Institute, University of Ulster, Coleraine, UK Introduction: Androgen-dependent prostate cancer is frequently

treated with androgen ablation therapy (AAT), however tumours often recur in 1 – 3 years with an aggressive, androgen-independent phenotype. It is proposed that treatment-induced Palbociclib cost stress factors in the tumour microenvironment, may contribute to this failure. Method: LNCaP tumours were grown on the backs of male SCID mice. Tumour oxygenation was measured before and (a) 24 hours after treatment with a panel of anti androgens (b) during 28 days of daily dosing with bicalutamide (2 mg/kg). LNCaP tumour fragments were implanted into a dorsal skin flap (DSF) onto the backs of SCID mice. The animals were treated with bicalutamide (2 mg/kg) daily and tumour vasculature was imaged weekly for 21 days. Results: Flutamide (25 mg/kg) and bicalutamide(10 mg/kg) significantly reduced tumour oxygenation after 24 hours.