883). (D) Significant correlation was found between plasma MMP-9 and circulating EPC levels for patients with ovarian cancer (P = 0.0027, r = 0.865). Discussion EPCs are considered bone-marrow derived RAD001 ic50 cells that migrate into the peripheral blood in response to cytokines such as VEGF [12]. In contrast to the ischemic condition, the role of circulating EPCs in tumor angiogenesis

and growth is unclear. EPCs possess a high proliferation potential and have been found to be a potential marker for both neovascularization and response to antiangiogenic therapies [13]. The role of EPCs in cancer angiogenesis and growth deserves further investigation, especially in regard to their potential as markers to monitor disease progression or treatment response. However, to the best of our knowledge, the potential effect of circulating EPCs in the progression and angiogenesis of ovarian cancer has not been reported. In the present study, we investigated the potential utility of circulating EPCs as a marker for ovarian tumor progression, angiogenesis, and prognosis. Previous studies demonstrated that EPCs levels in the peripheral

https://www.selleckchem.com/products/Adrucil(Fluorouracil).html blood of patients with breast cancer [14], non-small cell lung cancer [9], and lymphoma [15] were significantly higher compared with healthy volunteers. Similarly, we observed in the present study that the number of circulating EPCs was significantly higher in patients with ovarian cancer compared with healthy subjects. These findings support the results of animal studies regarding the mobilization and migration of bone marrow-derived EPCs via blood circulation into tumor neovasculature. Despite the small number of subjects in our study, we observed significant correlations between circulating EPCs levels and tumor the stage and residual tumor size in ovarian cancer patients. This was consistent with a previous study that reported the relationship between increased EPC levels and more advanced

stages of breast cancer [11]. We compared levels of EPCs in patients after surgery or chemotherapy treatment and found that both treatments reduced EPC levels, but not to the low level observed in healthy controls. Similarly, treatment was associated with a significant reduction in the levels of circulating EPCs in patients with lung cancer [9]. More importantly, follow-up revealed a significantly higher incidence of death from ovarian cancer in patients with high pre-treatment EPC levels compared with patients with low EPCs levels. These findings indicate a possible relationship between more aggressive ovarian cancer and higher circulating level of EPCs, suggesting that EPCs play a role in tumor growth and progression, thereby facilitating angiogenesis and metastasis. We next attempted to characterize EPCs-specific markers CD34 and VEGFR2 in the peripheral blood of patients with ovarian cancer by real-time RT-PCR.