Before purchased from Mediatech. Murine leukemia Mie-cell lines P388 and P388/ADR were obtained from the National Cancer Institute, Frederick. 2.2. Training PazPC / DOX complex L Solutions of ion pairs in DOX 20 mM HEPES buffer with different pH values of 4.5 9.0 from fra YEARS Made caspase Riger. PazPC in chloroform were used as films in Glasr Formed Hrchen under nitrogen and dried under vacuum. A predetermined volume of DOX L Solutions were min to the lipid film with R Hrchen added and hydrated at 60 ° C for 30 to a molar To obtain ratio of 1:1 PazPC / DOX. Streuintensit were t of mixtures of hydration using a Nicomp 380 Submicron Particle Sizer. The mixtures were centrifuged for 10 min at 10,000 rpm. The concentrations of DOX in the supernatant of the complex ion were measured fluorometrically at 480 nm and 590 nm. The proportion of ions drug was as follows paired calculated PazPC:% of the ion with drug PazPC assigned 100% To evaluate the influence of DOX L solubility to eliminate controlled experiments, the DOX L solutions used formed a precipitate and PazPC% were withdrawn in order net% ion paired with DOX PazPC receive. 2.3. Preparing PazPC / DOX or IDA was micelles in 100 l of chloroform PazPC pipette into a glass tube containing L Solvent was removed under a stream of nitrogen, and lipid films were kept under vacuum for 2 h. The dry lipid films were hydrated at 60 ° C for 30 min in 1 ml of DOX or IDA in 20 mM HEPES buffer to keep a 5:1 molar Ratio of PazPC / drugs. The drug-containing PazPC Mizellenl Solution was in a kind Ultraschallger Where t for 10 min. The prepared PazPC / DOX and PazPC / IDA micelles in subsequent experiments were used without further purification. Oxidized to an optimum pH for the formation of a complex between an ion pair phospholipid and DOX, Changes in the Streuintensit t and the resulting precip GE and mixtures PazPC DOX a function of pH was investigated to obtain.
As shown in Fig. 2A Streuintensit achieved a maximum at a pH of 7.0 7.5, and the current dropped to a pH of 7.0 or 7.5, as by the bell illustrated shaped curve. Similar results were obtained by centrifugation of mixtures PazPC and DOX. The proportion of the ions drug was coupled PazPC than 60% at pH 7.0 and 7.5, but it reduces fa Is spectacular Re at pH 7.0 or 7.5. Therefore, the pH 7.0, 7.5, the optimum pH range of complexes PazPC / DOX ion pair is formed. To the ratio Ratio of Irbesartan lipid drugs for the preparation of micelles loaded drug library with appropriate particle E and encapsulation efficiency to optimize the concentration constant and varying concentrations of DOX PazPCwere be used to hold micelles PazPC / DOX preparation. Changes in the charged particle E, intensity t dispersant, the encapsulation efficiency and the concentration of the drug PazPC / DOX micelles according to claim PazPC / DOX molar ratio In the ratio shown. 2B and C, if the ratio Ratio of PazPC / DOX gr As he is 2-1, the intensity t of mixtures PazPC / DOX significantly reduced compared to about 25 kHz, 170 kHz or 260 kHz. The particle E showed anything similar Ver Changes when the ratio Ratio gr It as 2:1. This shows that DOX loaded micelles PazPC by the interaction between ion-pair PazPC and DOX was formed when the ratio Ratio gr He than 2:1.