Data analysis All data was analyzed in SPSS using a mixed-factorial ANOVA [treatment (DBX vs PLC) x time Ibrutinib (HR1 vs HR2 vs HR3 vs HR4)]. Results REE and RER
A significant group x time interaction for change in resting energy expenditure (p = 0.001) was determined. From baseline to hour 4, REE increased by 147.33 ± 83.52 for DBX and 32.17 ± 86.72 kcal/day for PLC (p = 0.003). Changes in kcal/day for all time points can be seen in Figure 1. A significant main effect for time was also reported (p = 0.001). Changes in REE from baseline for each time point
are as follows: hour 1 (DBX: 123.4 ± 78.2 kcal/day vs. PLC: -3.1 ± 88.4 kcal/day), hour 2 (DBX: 125.5 ± 62.2 kcal/day vs. PLC: -20.3 ± 72.6 kcal/day), hour 3 (DBX: 142.4 ± 101.16 kcal/day vs. PLC: 9 ± 114.77 kcal/day), and hour 4 (DBX: 147.3 ± 83.5 kcal/day vs. PLC: 32.1 ± 86.7 kcal/day). Changes were significant (p < .05) between groups at all time points for REE. There were no significant time or interaction effects for RER at any time point. Figure 1 Resting energy expenditure changes. REE increased across all time points for DBX (active) ranging from a 123.4 to 147.3 SCH772984 manufacturer kcal/day increase above baseline values. Changes were statistically different between groups at all time points post-supplementation. * indicates statistically significant changes (p ≤ 0.05). Hemodynamic and ECG There were no significant FER (p > 0.05) group x time interactions and
no main effects for time for SBP, DBP, or HR (Figure 2). There was no significant main effect for group (p > 0.05). At hour 1, SBP increased by 12.4 ± 11.8 mmHG and 1.75 ± 10.4 mmHG for DBX and PLC, respectively from baseline values. From baseline to hour 2, SBP increased by 10.0 ± 14.0 mmHg (DBX) versus 0.0 ± 7.9 mmHg (PLC). Hour 3 SBP deviated from baseline by 13.5 ± 22.4 mmHg for DBX and −2.5 ± 8.1 mmHg for PLC. Hour 4 SBP increased above the baseline mean by 8.3 ± 10.5 mmHg (DBX) and 1.5 ± 10.6 mmHg (PLC). DBP changes from baseline to hour 1 were 4.8 ± 7.4 mmHg (DBX) versus 0.6 ± 7.9 mmHg (PLC). At hour 2, DBP changed from baseline by −0.25 ± 13.2 (DBX) and −1.0 ± 7.2 mmHg (PLC). Hour 3 values for DBP from baseline for DBX were 6.7 ± 20.9 mmHg and for PLC were −4.5 ± 10.1 mmHg. The comparison against DBP baseline measurement for the DBX group at hour 3 was 1.25 ± 6.8 mmHg and 1.1 ± 11.0 mmHg for the PLC group. DBX versus PLC comparison to baseline in HR are as follows: hour 1 (−3.0 ± 6.2 vs. -2.5 ± 5.5 bpm), hour 2 (−2.9 ± 6.5 vs. -1.0 ± 10.0 bpm), hour 3 (−2.3 ± 5.6 vs. -0.5 ± 8.7 bpm), and hour 4 (−1.4 ± 6.8 vs. -0.3 ± 7.4 bpm). (Data can be seen in Table 2 for SBP, DBP, and HR.