(DOC 70 KB) Additional file 2: Supplementary Figure 1: Maximum li

(DOC 70 KB) Additional file 2: Supplementary Figure 1: Maximum likelihood inference phylogeny based

on the concatenated MLST data, 2,079 bp. (Please note that tree has been rooted to find more the supergroup D sequences). (JPG 99 KB) Additional file 3: Supplementary Figure 2: Maximum likelihood inference phylogeny based on the on the wsp sequence. (Please note that tree has been rooted to the supergroup D sequences). (JPG 124 KB) References 1. Werren JH: Biology of Wolbachia . Annu Rev Entomol 1997, 42:587–609.PubMedCrossRef 2. Werren JH, Windsor DM: Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proc Biol Sci 2000,267(1450):1277–1285.PubMedCrossRef 3. Jeyaprakash A, Hoy MA: Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Insect Mol Biol 2000,9(4):393–405.PubMedCrossRef 4. Hilgenboecker K, Hammerstein

P, Schlattmann P, Telschow A, Werren JH: How many species are infected with Wolbachia ?-A statistical analysis of current data. FEMS Microbiol Lett 2008,281(2):215–220.PubMedCrossRef 5. Bandi C, Anderson TJ, Genchi C, Blaxter ML: Phylogeny of Wolbachia in filarial nematodes. Proc Biol Sci 1998,265(1413):2407–2413.PubMedCrossRef 6. Bourtzis K, O’Neill BAY 80-6946 datasheet S: Wolbachia infections and arthropod reproduction – Wolbachia can cause cytoplasmic incompatibility, parthenogenesis, and feminization in many arthropods. Bioscience 1998,48(4):287–293.CrossRef 7. Werren JH, Baldo L, Clark ME: Wolbachia : master manipulators of invertebrate biology. Nat Rev Microbiol 2008,6(10):741–751.PubMedCrossRef 8. Stouthamer R, Breeuwer JA, Hurst GD: Wolbachia pipientis : microbial manipulator of arthropod reproduction. Annu Rev Microbiol 1999, 53:71–102.PubMedCrossRef 9. Bourtzis K, Miller TA: Insect Symbiosis. Florida, USA: CRC Press; 2003.CrossRef 10. Saridaki A, Bourtzis K: Wolbachia : more than just a bug in insects genitals.

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