If it is proven to be true, Guaifenesin would be a suitable candidate as an antiepileptic courtesy of its wide margin of safety. Guaifenesin can also be used in pregnancy and breastfeeding,15,16 in contrast to other antiepileptic drugs. The aim of the present study was to determine whether Guaifenesin has an anticonvulsant effect in an animal model of seizure. Materials and Methods Chemicals Guaifenesin was purchased from Exir Pharmaceutical Company, Iran and was dissolved in 0.25% Tween 20 in normal saline. Diazepam (10 mg/ml)

was obtained from Darupakhsh Pharmaceutical Company, Iran, and Pentylenetetrazol (PTZ) was purchased from Sigma and was dissolved Inhibitors,research,lifescience,medical in normal saline. Animals Male albino mice (25-35 g) were obtained from the Animal House, Shiraz University of Medical Sciences. The animals were kept Inhibitors,research,lifescience,medical in plastic cages in groups of 4-5 with free access to food and water, 12h dark/12h light cycles, and at a temperature of 22ºC±2. The animals were treated in accordance with the guidelines of the Ethics Committee of Shiraz University of

Medical Sciences. PTZ-Induced Seizure The anticonvulsant effect of Guaifenesin was assessed in an animal model of PTZ-induced seizure. Sixty mice were randomly assigned into 6 groups (n=10/group) and received Inhibitors,research,lifescience,medical Guaifenesin intraperitoneally at doses of 100, 200, 300, and 400 mg/kg 30 minutes before the injection of PTZ (95 mg/kg). The control group was administered 0.25% Tween in normal saline. A group of mice received Diazepam (3 mg/kg) as a reference drug. The doses of Guaifenesin and Diazepam were selected according to previous studies.17,18 The latency Inhibitors,research,lifescience,medical time to the onset of myoclonic, clonic, and tonic-clonic convulsions was recorded after PTZ injection. PTZ initially produced myoclonic jerks, followed by facial and forelimb clonus, which then became sustained and led to generalized tonic-clonic Inhibitors,research,lifescience,medical jerking (loss of righting reflex and tonic forlimb flexion/extension, followed by whole body clonus).19 In addition, the percentage of the animals exhibiting convulsion and the percentage of mortality were recorded for each

studied group. The ED50s, median effective doses of Guaifenesin, protecting 50% of the mice Dacomitinib against clonic and tonic-clonic convulsions and death were determined. Neuromuscular Coordination–Rotarod The effect of Guaifenesin on coordinated motor movements was assessed using the Rotarod test. A day before the test, the mice were trained to stay on the rotating wheel (3 cm in diameter, 20 rpm) for more than one minute. On the test day, the animals were figure 1 tested on the Rotarod (model 7600, UGO Basile, Italy) before and 30 minutes after the administration of 0.25% Tween20, Diazepam or different doses of Guaifenesin. The number of seconds each mouse remained on the rotating wheel was recorded for a maximum of 300 seconds. Statistical Analysis The data are presented as mean value±SEM.