Logistic regression analysis was used to examine risk factors for complications related to PD and to compare mortality between ARF and non-ARF patients controlling for potential confounding factors. A total of 43 complications related to PD were registered in 27 (20.8%) patients. Major complications were seen in eight (6.2%) patients, and only two (1.5%) patients were switched to
hemodialysis after peritonitis and hemicolectomy due to bowel perforation. The main risk factors for complications to PD were duration of PD, high RACHS-1 score (Risk Adjusted Classification for Congenital Heart Surgery), and hyperkalemia at initiation of PD. Overall, in-hospital mortality was 6.8% (76/1128). Mortality of ARF patients was 20.0% compared to 5.0% among non-ARF patients (adjusted odds ratio
= 1.91, 95% confidence interval = 1.10 – 3.36). After stratification, ARF was strongly Selleck Sapitinib associated with increased mortality in the subgroups of patients with the lowest overall risk of dying (age >= 1 year, body weight >= 5 kg, RACHS-1 score <3, and no preoperative cyanosis). For patients at high risk of dying (age <1 year, body weight <5 kg, RACHS-1 score >= 3, cardiopulmonary bypass time >= 60 min, and preoperative cyanosis), the association Cytoskeletal Signaling inhibitor between ARF and mortality was substantially weaker. In conclusion, postoperative ARF was associated with increased mortality in children operated for congenital heart disease. Major complications to PD were few, and our data strongly support that PD is a simple, safe, feasible, and robust dialysis modality for the management of ARF in children.”
“To investigate peroxidase
PRT062607 mw induced 3 ‘-nitrotyrosine (3NT) formation, neutrophil derived myeloperoxidase (MPO) (0.025 mu M) was directly administered to A549 epithelial cells with or without H2O2 (150 mu M). Little evidence of 3NT was found. In contrast, there was a dose dependent increase in intracellular NO (p < 0.001, n = 
following MPO (0.025 mu M) treatment, which was further enhanced (p < 0.0003, n = by addition of H2O2. Extracellular NO also increased after MPO (p < 0.002, n = 5) and with MPO and H2O2 (p < 0.004, n = 5). Substantial 3NT formation was only detected following addition of nitrite (NO2-, >= 100 mu M), which induced a dose dependent increase in epithelial 3NT. In contrast, protein carbonyl formation and increased GSSG/GSH ratios were associated with MPO treatment even in the absence of NO2-. CO-Culture of A549 epithelial cells with polymorphonuclear leukocytes (PMN) (10(6)/ml) led to immunocytochemical detection of epithelial 3NT and induction of nitric oxide synthase (NOS2). However, in a Transwell system direct contact between PMN and A549 cells was necessary for immunodetection of 3NT but not of NOS2 consistent with a role for high local nitrite concentrations. These findings demonstrate dissociation between epithelial endogenous NO production and 3NT formation.