Otal hamster microsomes LY335979 P-glycoprotein inhibitor , or delivery of drugs, as described in the experimental section. The livers were removed and homogenized, and total microsomes isolated. The proteins Were tested and the lipids were extracted from aliquots of homogenates and microsomes and quantitative HPTLC ? ed. Results Mean values SD of six experiments are ?. Student was used t-test to compare the values of the experimental samples on the manipulated S compare, P! 0.01, P! 0.002, P unmarked values 005th Ed protein Unesteri ? Cholesterol Cholesterol Cholesterol ester phospholipids TAG homogenate fed ? 230.07 68.036 2.89 0.19 2.02 0.19 33.84 0.80 317.94 66.11 210.95 fed ?? ? ? Chow? ? 14.36 2.48 0.64 4.42 0.12 2.59 0.18 253.01 27.40 229.56 15.87 1.52 ? ? ? simvastatin treated ? ? 0.27 5 09 0.
36 0.43 302.20 PHA-680632 67.26 0.06 ? ? ? ACAT inhibitor ? cholesterol 213.01 12.76 1.57 0.06 9.34 0.58 0.31 0 ?? ??fed 06 308.41 53.10 20.30 2.48 0.55 ? microsomal cholesterol ? ? 0.04 1.11 0.18 0.271 0.063 19.95 3.32 ? ? ? Chow fed 24.80 4.43? ? 0.67 0.13 1.75 0.31 0.174 0.034 20.97 3.69 ? ? ? simvastatin treated ? 26.65 2.63 0.56 0.12 3.23 ? ? 0, 78 0,? 0597 0.019 22.76 2.32 ? ? ACAT inhibitor ? cholesterol ? ? 27.53 1.12 0.69 0.02 2.75 0.98 0.0839 0.005 20.02 1 ?, 07 ? lipid composition table 2 microsomal membranes from the liver of hamsters subjected Ern currency or medicine se treatment Total microsomes from the liver of hamsters were made. microsomal vesicles were separated into fractions of membrane and luminal contents and the lipids were extracted and analyzed by HPTLC, as described in the experimental section.
The results are means for the composition of the membrane ? SD of six experiments. Student t-test was used to the experimental sample with the value fed chow compare P embroidered! 0.01, P! 0.002, sch protected Unchecked “P 0.05. Unesteri ? cholesterol esters ed DAY Cholesterol Cholesterol 16.79 1.22 39.25 6.13 3.12 0.42 ? ? ? fed chow fed 14.81 3 63 31 , 68 4.71 1.97 0.34 ? ? ? simvastatin contract 13.23 36.11 2.34 0.91 0.19 7.8 cholesterol ? ? ? ACAT inhibitor ? 15.25 1 99 29.83 4.02 1.18 0.23 ? ? ? term HMG-CoA reductase and LDLr in the liver of hamsters subjected Ern currency or di t cholesterol drug se treatment reduces the H see the mRNA of HMG-CoA reductase and LDLr 35% and 15% embroidered on to chow fed, compared w during treatment with simvastatin or ACAT inhibitor of cholesterol ? hte increased mRNA levels of HMG-CoA reductase, 362% and 212%, and the LDLr 188% and 186%, respectively.
HMG-CoA activity t in liver microsomes showed a relatively large e variation between individual hamsters, but there was decreased not significantly ? eo C60% activity t in liver microsomes from hamsters fed cholesterol and increased hte activity th of 27 and 14 times treated, both in microsomes of hamsters with simvastatin or ? ACAT inhibitor of cholesterol. SREBP 2 Breakdown by ER gradient fractions from the livers of hamsters subjected to various Di th or drugs, the distribution of SREBP 2 in various To determine compartments of the ER prepared were the hamster liver microsomes # 2001 Biochemical Society 418 CR Iddon and Table 3 Effect of other foods or medicine se treatment on the expression of HMG-CoA reductase and LDLr mRNA levels in the liver of hamster HMG- CoA reductase and LDLr were determined by testing the RNase protection, as described under Experimental methods. T