, thickness, buy I-BET151 weight variation, folding endurance, loss of moisture, moisture uptake and drug content.13 The results were given in Table 2. Thickness of the patches was measured using a screw gauge at different places of the patch and average thickness was determined. Weight of three individual patches of 2 × 2 cm2 was determined and average weight was calculated. Folding endurance was determined by folding and opening the patch at the same place repeatedly until a break developed at the place of folding. The value was expressed

as a number that indicates the number of times the patch was folded to develop a break. Patches of 1 × 1 cm2 were weighed individually and kept in a dessicator containing calcium chloride at room temperature for 24 h. The final weight was noted when there was no further change in the weight of individual patch. The percent moisture loss was calculated as difference between initial Cabozantinib manufacturer and final weight with respect to final weight. Patches of 1 cm2 were taken for drug content estimation. Transdermal patch of 1 cm2 was cut into small pieces and triturated for 30 min, to facilitate better extraction of drug. The contents were transferred into a 10 ml volumetric flask and the mortar was rinsed with small portion of 0.1% sodium

hydroxide and was also transferred to volumetric flask. The solution was shaken for 30 min and was filtered through whatmann-1 filter paper and the filtrate was examined for the drug content at 254 nm. In vitro diffusion studies were done using Franz diffusion cell having a diffusion area of 4.89 cm2. Phosphate Linifanib (ABT-869) buffer pH 7.4 was taken in receptor compartment at room temperature with dialysis membrane separating the two compartments. Drug loaded patch was placed on the dialysis membrane and the donor compartment was clamped on this assembly. The contents of the receptor compartment were stirred continuously at about 100 rpm using a magnetic bead. 5 ml of the buffer from the receptor medium was removed at regular intervals, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 24 h and was replaced with

equal volume of fresh buffer to maintain sink condition. The withdrawn samples were appropriately diluted and measured on UV-spectrophotometer at 254 nm. UV spectroscopic method was developed for losartan potassium in phosphate buffer pH 7.4 and validated. The analytical wave-length of 254 nm was identified and is similar to literature report.12 Beer-Lambert’s law was obeyed (R2 = 0.9997). All prepared films were clear, transparent, flexible and smooth. Formulations LP-1 to LP-4 were found to be sticky and higher moisture content was also observed on account of high plasticizer concentration. The physicochemical properties were found to be within limits. The values were given in Table 2. From the results, it was observed that when the polymer PMMA was high in LP-6 and LP-12 and LP-13, average weight, thickness increased.