We discussed the control of the amount of FePt nanoparticles and PDDA molecules is absolutely imperative to obtain the thin shell and the whole diameter suitable for nano-DDS and demonstrated sufficient amount of FePt nanoparticles and PDDA molecules to obtain 3D structure. Especially selleck chemicals llc in the FePt-nanoparticle/PDDA hybrid shell, FePt nanoparticles anchor the PDDA molecules in an aqueous solution, therefore the amounts of PDDA molecules adsorbed on the silica particles and FePt nanoparticles accumulated on the PDDA layer were important to maintain the structure of the shell. FePt network capsules loaded with anti-cancer drugs and coated with lipid membrane to avoid leaks of that drugs showed cellular toxicity to gastric cancer.

Results and Discussion Morphologies Composite particles and hybrid capsules FePt nanoparticles were synthesized at 503 K in the presence of silica particles treated with various concentrations of PDDA aqueous solution (1�C7 wt%) by reduction of Fe(acetylacetonate)3 (0.106 mmol) and Pt(acetylacetonate)2 (0.096 mmol).26,27 The modification of negatively charged silica template particles with a cationic polymer resulted in the zeta potential of the silica template particles changing from negative to positive. The adsorption of PDDA molecules on the surface of silica particles was confirmed by measuring their zeta potentials. Increasing the concentration of the PDDA solution used to treat the silica particles, the morphologies of the composite particles fabricated with these PDDA-modified silica particles were slightly different (Fig. 1A�CC).

FePt nanoparticles accumulated on the surface of the PDDA-modified silica particles, and there was no difference in the size and shape of these FePt nanoparticles; the diameter of the FePt nanoparticles was between 3 and 5 nm. However, silica template particles treated with lower concentrations of PDDA solution (1 or 5 wt%) were only partially covered with FePt nanoparticles, whereas silica particles treated with 7 wt% PDDA solution were entirely covered with FePt nanoparticles (Fig. 1A�CC). Our previous report demonstrated that FePt nanoparticles accumulate on organic compounds adsorbed on the surface of silica particles18 confirming that the surface of the silica template particles treated with 1 or 5 wt% PDDA solution were not entirely covered with PDDA molecules, and that FePt nanoparticles accumulated only at the PDDA molecules adsorbed on the silica surface.

This means that the FePt nanoparticles were selectively grown on the PDDA layer. Figure 1D�CF shows TEM images of FePt-nanoparticle/PDDA hybrid capsules fabricated by dissolution of the silica template particles from the composite particles shown in Figure 1A�CC. Complete FePt-nanoparticle/PDDA hybrid capsules were successfully obtained by dissolving the silica template particles from the composite particles entirely covered with FePt nanoparticles (Fig. Anacetrapib 1F).