We found highly correlated
protein abundance ratios, indicating that the LTQ-Orbitrap plus our technique yields results comparable to the current standard. We implemented our technique in a freely available, automated software pipeline, called LTQ-iQuant, which is mzXML-compatible; supports iTRAQ 4-plex and 8-plex LTQ data; and can be modified for and have weights trained to a user’s LTQ and other isobaric peptide tagging VE-822 ic50 methods. LTQ-iQuant should make LTQ instruments and isobaric peptide tagging accessible to more proteomic researchers.”
“Introduction: [F-18]FDG PET has difficulty distinguishing tumor from inflammation in the clinic because of the same high uptake in nonmalignant and inflammatory tissue. In contrast, amino acid tracers do not accumulate in inflamed tissues
and thus provide an excellent opportunity for their use in clinical cancer imaging. In this study, we developed a new amino acid tracer 5-(3-[F-18]Fluoropropyloxy)-L-tryptophan ([F-18]-L-FPTP) by two-step DihydrotestosteroneDHT supplier reactions and performed its biologic evaluation.
Methods: [F-18]-L-FPTP was prepared by [F-18]fluoropropylation of 5-hydroxy-L-tryptophan disodium salt and purification on C18 cartridges. The biodistribution of [F-18]-L-FPTP was determined in normal mice and the incorporation of [F-18]-L-FPTP into tissue proteins was investigated. In vitro competitive inhibition experiments Farnesyltransferase were performed with Hepa1-6 hepatoma cell lines. [F-18]-L-FPTP PET imaging was performed on tumor-bearing and inflammation mice and compared with [F-18]-L-FEHTP PET.
Results: The overall uncorrected radiochemical yield of [F-18]-L-FPTP was 21.1 +/- 4.4% with a synthesis time of 60 min, the radiochemical purity was more than 99%. Biodistribution studies demonstrate
high uptake of [F-18]-L-FPTP in liver, kidney, pancreas, and blood at the early phase, and fast clearance in most tissues over the whole observed time. The uptake studies in Hepa1-6 cells suggest that [F-18]-L-FPTP is transported by the amino acid transport system B-0,B-+, LAT2 and ASC. [F-18]-L-FPTP displays good stability and is not incorporated into proteins in vitro. PET imaging shows that [F-18]-L-FPTP can be a better potential PET tracer for differentiating tumor from inflammation than [F-18]FDG and 5-(3-[F-18]fluoroethyloxy)-L-tryptophan ([F-18]-L-FEHTP), with high [F-18]-L-FPTP uptake ratio (2.53) of tumor to inflammation at 60 min postinjection.
Conclusions: Using [F-18]fluoropropyl derivatives as intermediates, the new tracer [F-18]-L-FPTP was achieved with good yield and radiochemical purity, and the biological evaluation results of [F-18]-L-FPTP showed that it was a hopeful tracer for PET tumor imaging. (C) 2013 Elsevier Inc. All rights reserved.”
“Background: Simultaneous treatment of multilevel aortic disease is controversial due to the theoretic increase in morbidity.