To determine infiltration of selleck inhibitor amoebae into the deep layers of muscle tissues, surface layers were removed and only deep tissues were sectioned. Trophozoites

of amoebae (indicated by arrows) were detected in these tissue samples on days 5 (C) and 7 (D) post-infection, but not on day 3 (B), nor in control muscles (A). Degenerative changes of the tissues caused by the amoebae are significant on days 5 and 7. Note that the above images are representative micrographs of the genotype T4, but, similar results were observed with the T1 genotype. Magnification is × 400. (ii) Fat body surrounding the brain of Acanthamoeba-injected locusts was examined on days 3, 5 and 7 after Vemurafenib research buy injection. A large numbers of amoebae (pointed by arrowheads) were identified in the fat body on days 5 (C) and 7 (D) after injection. However, they

were found in much fewer numbers on day 3 (B). No amoeba was observed in the fat body of control locusts (A). Note that the above images are representative micrographs of the genotype T4, but, similar results were observed with the T1 genotype. Magtnification is × 400. Amoebae were observed not only in the brain of infected locusts, but also in the suboesophageal ganglion. Both isolates of Acanthamoeba infiltrated this ganglion and caused noticeable histopathological damage (Fig. GSK461364 nmr 5i). Occasionally, sporadic amoebae in the form of trophozoites were seen also in the lumen of the foregut of some of Rebamipide the infected locusts (Fig. 5ii), but this was not a consistent finding in all foreguts sectioned. Figure 5 Acanthamoeba invades the locust suboesophageal ganglion and occasionally present in the lumen of the locust foregut. (i) (A) Suboesophageal ganglia of control locust (×100); (B) Suboesophageal ganglia of infected locust (×100); (C) Suboesophageal ganglia of control locust at higher

magnification (×400); (D) Suboesophageal ganglia of infected locust at higher magnification (×400). Note that the trophozoites of amoeba (indicated by arrowheads in D) were detected in the suboesophageal ganglion of infected locusts, but not in control locusts (C). Histopathological damage in suboesophageal ganglia from infected locusts (B) was also evident, when compared with the ganglia in control locusts (A). The above images are representative micrographs of the genotype T4, but, similar results were observed with the T1 genotype. (ii) (A) Foregut of control locust (×250); (B) Foregut of infected locust (×250); (C) Foregut of infected locust at higher magnification (×400). Trophozoites of amoeba (indicated by arrow in C) were identified in the lumen of the foregut of some of the locusts. No damage to the wall of the foregut was observed in the infected locusts. Note that the above images are representative micrographs of the genotype T4, but, similar results were observed with the T1 genotype.