Expression of 1 OHase is expression with cAMP treatment in these patients H&E stain shows the two most common cell types of the parathyroid gland: large, eosinophilic oxyphil cells and the smaller chief cells. An adjacent section immunostained for 1 OHase Osthole shows expression is highly expressed in oxyphil cells compared to chief cells. Representative images; magniﬁcation. Quantitation of 1 OHase immunostaining shows a signiﬁcant 7-fold increase in expression in parathyroid oxyphil cells. Results reported as integrated optical density per unit area . C.S. Ritter et al. / Journal of Steroid Biochemistry & Molecular Biology 73– Effects of cAMP or FGF-23 on 1 OHase mRNA in human parathyroid cells.
Parathyroid cells from patients with secondary hyperparathyroidism cultured in suspension with or without 2 mM dibutyryl-cAMP for 1 h resulted in a signiﬁcant, 53% increase in 1 OHase mRNA. Data reported as average SEM, percent of control values . Parathyroid cells from patients with secondary hyperparathyroidism were cultured in Ecdysone suspension with or without ng/ml FGF-23 for 1 h, resulting in a signiﬁcant, 8-fold increase in 1 -OHase mRNA. Data reported as average SEM, percent of control value . 0), under these conditions. Treatment of parathyroid cells with or without ng/ml FGF-23 for 1 h, resulted in a 8-fold increase in 1 OHase mRNA, as shown in 4 B There was no change in PTH mRNA expression with FGF-23 treatment .
The effect of calcium on 1 OHase and PTH mRNA in human parathyroid cells was examined. Monolayers were treated for 24 h with LC or HC medium; cells from 2 of these patients were also treated with NC medium. As shown in 5 , HC produced a 53% increase in 1 OHase mRNA compared to LC treatment; there was no signiﬁcant difference in the purchase LY450139 expression of 1 OHase between LC and NC treatment . HC treatment resulted in a 59% decrease in PTH mRNA; the expression of PTH was similar with LC and NC treatment . In addition to calcium, the effect of the calcium receptor activator cinacalcet on 1 OHase and PTH mRNA expression in parathyroid cells was examined. Monolayers were Effect of calcium on 1 OHase and PTH mRNA in human parathyroid cells.
Parathyroid cell monolayers from a patient with secondary hyperparathyroidism were treated for 24 h with low calcium , normal calcium or high calcium medium . HC conditions produced a 53% increase in 1 OHase mRNA compared to LC , and a 59% decrease in order Cidofovir PTH mRNA . Results reported as average SEM, percent of LC values . treated 24 h with medium containing normal calcium with or without cinacalcet ; ethanol served as vehicle control. As shown in 6 , cinacalcet treatment resulted in a 42% increase in expression of 1 OHase, compared to the NC control , and a 53% decrease in PTH mRNA . Regulation of 24OHase mRNA in human parathyroid cells We also investigated nanotechnology the regulation of the vitamin D metabolizing enzyme 24O Hase in human parathyroid cells. As shown in 7 , treatment of monolayers with HC result.